Cloning and sequencing of the sacA gene: characterization of a sucrase from Zymomonas mobilis
| Autor: | Brigitte Cami, L. Preziosi, Jacques C. Baratti, Paramasamy Gunasekaran, T. Karunakaran, A. G. Mukundan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Glycoside Hydrolases
Molecular Sequence Data Restriction Mapping Gene Expression Molecular cloning Regulatory Sequences Nucleic Acid medicine.disease_cause Microbiology Zymomonas mobilis Sucrase medicine Consensus sequence Escherichia coli Amino Acid Sequence Cloning Molecular Codon Molecular Biology Gene Peptide sequence Gram-Negative Anaerobic Bacteria biology Base Sequence beta-Fructofuranosidase Nucleic acid sequence biology.organism_classification Biochemistry Research Article |
| Popis: | The Zymomonas mobilis gene (sacA) encoding a protein with sucrase activity has been cloned in Escherichia coli and its nucleotide sequence has been determined. Potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to E. coli and Z. mobilis consensus sequences. Extracts from E. coli cells, containing the sacA gene, displayed a sucrose-hydrolyzing activity. However, no transfructosylation activity (exchange reaction or levan formation) could be detected. This sucrase activity was different from that observed with the purified extracellular protein B46 from Z. mobilis. These two proteins showed different electrophoretic mobilities and molecular masses and shared no immunological similarity. Thus, the product of sacA (a polypeptide of 58.4-kDa molecular mass) is a new sucrase from Z. mobilis. The amino acid sequence, deduced from the nucleotide sequence of sacA, showed strong homologies with the sucrases from Bacillus subtilis, Salmonella typhimurium, and Vibrio alginolyticus. |
| Databáze: | OpenAIRE |
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