Cell remodeling and subtilase gene expression in the actinorhizal plant Discaria trinervis highlight host orchestration of intercellular Frankia colonization
| Autor: | Nacira Muñoz, Hernán Ramiro Lascano, Sergio Svistoonoff, Leandro Imanishi, Valérie Hocher, Joëlle Fournier, David G. Barker, Elodie Pirolles, Lukas Brichet, Iltaf Abdou-Pavy, Luis Gabriel Wall, Mireille Chabaud, Laurent Brottier, Hassen Gherbi, Alice Vayssières, Andrea Genre |
|---|---|
| Přispěvatelé: | Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Universidad Nacional de Quilmes (UNQ), Department of Life Sciences and Systems Biology [University of Turin], University of Turin, Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Diversité, adaptation, développement des plantes (UMR DIADE), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), French National Research Agency : ANR-12-BSV7-0007-02, National University of Quilmes, Argentina : 0395/07 1411/15, Argentinian National Council for Scientific & Technical Studies (CONICET) : PIP 2271, French National Research Institute for Sustainable Development (IRD-DPF), ECOS-SUD : A07B02, A13B03, CONICET (Argentina), 'TULIP' Laboratory of Excellence : ANR-10-LABX-41, ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Department of Life Sciences and Systems Biology, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Physiology intercellular colonization Frankia Colony Count Microbial Plant Science Root hair Models Biological actinorhizas Rhizobia 03 medical and health sciences subtilisin-like serine protease Gene Expression Regulation Plant Plant Cells Compartment (development) subtilisin‐like serine protease [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Colonization Subtilisins Promoter Regions Genetic biology Host (biology) Rhamnaceae fungi biology.organism_classification Apoplast Cell biology 030104 developmental biology apoplastic remodeling nitrogen fixation endosymbiotic infection Discaria trinervis Root Nodules Plant Actinorhizal plant |
| Zdroj: | New Phytologist New Phytologist, Wiley, 2018, 219 (3), pp.1018-1030. ⟨10.1111/nph.15216⟩ |
| ISSN: | 0028-646X 1469-8137 |
| DOI: | 10.1111/nph.15216⟩ |
| Popis: | International audience; Nitrogen-fixing filamentous Frankia colonize the root tissues of its actinorhizal host Discaria trinervis via an exclusively intercellular pathway. Here we present studies aimed at uncovering mechanisms associated with this little-researched mode of root entry, and in particular the extent to which the host plant is an active partner during this process. Detailed characterization of the expression patterns of infection-associated actinorhizal host genes has provided valuable tools to identify intercellular infection sites, thus allowing in vivo confocal microscopic studies of the early stages of Frankia colonization. The subtilisin-like serine protease gene Dt12, as well as its Casuarina glauca homolog Cg12, are specifically expressed at sites of Frankia intercellular colonization of D. trinervis outer root tissues. This is accompanied by nucleo-cytoplasmic reorganization in the adjacent host cells and major remodeling of the intercellular apoplastic compartment. These findings lead us to propose that the actinorhizal host plays a major role in modifying both the size and composition of the intercellular apoplast in order to accommodate the filamentous microsymbiont. The implications of these findings are discussed in the light of the analogies that can be made with the orchestrating role of host legumes during intracellular root hair colonization by nitrogen-fixing rhizobia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text