FoxO inhibits juvenile hormone biosynthesis and vitellogenin production in the German cockroach
| Autor: | José L. Maestro, Marc Abrisqueta, Songül Süren-Castillo |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), European Commission, Consejo Superior de Investigaciones Científicas (España) |
| Rok vydání: | 2012 |
| Předmět: |
Hydroxymethylglutaryl-CoA Synthase
medicine.medical_specialty endocrine system DNA Complementary animal structures Fat Body Molecular Sequence Data Vitellogenin Biology Real-Time Polymerase Chain Reaction Biochemistry Vitellogenins Internal medicine medicine Animals Insulin RNA Messenger Molecular Biology Transcription factor PI3K/AKT/mTOR pathway Phylogeny RNA Double-Stranded Gene knockdown Reproduction Forkhead Transcription Factors Blattellidae Juvenile Hormones Insulin receptor Endocrinology Blattella germanica Gene Expression Regulation Insect Science Nutritional signalling Juvenile hormone biology.protein Female Hydroxymethylglutaryl CoA Reductases RNA Interference FoxO Corpus allatum Food Deprivation Sequence Alignment |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0965-1748 |
| Popis: | The transcription factor Forkhead-box O (FoxO) is the main transcriptional effector of the Insulin Receptor/Phosphatidylinositol 3-kinase (InR/PI3K) pathway. In a situation of nutrient restriction, the pathway is inactive and FoxO translocates to the nucleus to exert its transcriptional action. In starved females of the cockroach . Blattella germanica, the reproductive processes, and in particular the synthesis of juvenile hormone in the corpora allata and that of vitellogenin in the fat body, are arrested. In the present report we examine the possible role of FoxO in the transduction of the nutritional signals to these reproductive events. We first cloned FoxO cDNA from . B. germanica (BgFoxO), and showed that its expression is not nutritionally regulated. BgFoxO knockdown using systemic RNAi . in vivo in starved females elicited an increase of juvenile hormone biosynthesis, although without modifying mRNA levels of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase-1, HMG-CoA synthase-2, HMG-CoA reductase or methyl farnesoate epoxidase (CYP15A1) in corpora allata. In addition, BgFoxO RNAi treatment produced a remarkable increase of vitellogenin mRNA levels in fat body and of vitellogenin protein in the haemolymph. Our results indicate that BgFoxO plays an inhibitory role on juvenile hormone biosynthesis and vitellogenin production in a situation of nutrient shortage. © 2012 Elsevier Ltd. This work was supported by grants BFU2006-01090/BFI (Spanish Ministry of Science and Innovation (MICINN) and FEDER) and BFU2010-15906 (MICINN) to J.L.M. M.A. and S. S.-C. are recipients of a pre-doctoral fellowship (MICINN) and a post-doctoral contract (CSIC, JAE program co-funded by the European Social Fund), respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect