Humoral and cellular immune responses to Yersinia pestis Pla antigen in humans immunized with live plague vaccine
| Autor: | L. V. Sayapina, Onega V. Ulianova, Anna Lyapina, Vladimir L. Motin, Sergey S. Zaitsev, Svetlana A. Lebedeva, Valentina A. Feodorova, Maxim V. Telepnev, Tatiana E. Arseneva, Alexey L. Trukhachev, Maria A. Khizhnyakova, Sergey S. Ulyanov, Elena P. Lyapina |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Vaccines Live Unattenuated lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Virulence Factors Yersinia pestis 030106 microbiology Epitope 03 medical and health sciences Plasminogen Activators Immune system Antigen Bacterial Proteins Immunity Humans Aged Antigens Bacterial Immunity Cellular Plague Plague Vaccine biology lcsh:Public aspects of medicine Vaccination Public Health Environmental and Occupational Health lcsh:RA1-1270 Middle Aged biology.organism_classification Virology Immunity Humoral 030104 developmental biology Infectious Diseases Immunoglobulin G biology.protein Plague vaccine Cytokines Epitopes B-Lymphocyte Th17 Cells Female Antibody Biomarkers |
| Zdroj: | PLoS Neglected Tropical Diseases, Vol 12, Iss 6, p e0006511 (2018) |
| ISSN: | 1935-2735 |
| Popis: | Background To establish correlates of human immunity to the live plague vaccine (LPV), we analyzed parameters of cellular and antibody response to the plasminogen activator Pla of Y. pestis. This outer membrane protease is an essential virulence factor that is steadily expressed by Y. pestis. Methodology/Principal findings PBMCs and sera were obtained from a cohort of naive (n = 17) and LPV-vaccinated (n = 34) donors. Anti-Pla antibodies of different classes and IgG subclasses were determined by ELISA and immunoblotting. The analysis of antibody response was complicated with a strong reactivity of Pla with normal human sera. The linear Pla B-cell epitopes were mapped using a library of 15-mer overlapping peptides. Twelve peptides that reacted specifically with sera of vaccinated donors were found together with a major cross-reacting peptide IPNISPDSFTVAAST located at the N-terminus. PBMCs were stimulated with recombinant Pla followed by proliferative analysis and cytokine profiling. The T-cell recall response was pronounced in vaccinees less than a year post-immunization, and became Th17-polarized over time after many rounds of vaccination. Conclusions/Significance The Pla protein can serve as a biomarker of successful vaccination with LPV. The diagnostic use of Pla will require elimination of cross-reactive parts of the antigen. |
| Databáze: | OpenAIRE |
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