Design of a multiplex PCR method for detection of toxigenic-pathogenic in Vibrio cholerae
| Autor: | Ali Karami, Islamieh D Iman, Fooladi A A Imani, Seyed Mahmoud Amin Marashi, Doust R Hosseini |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Rapid detection Virulence Iran Biology urologic and male genital diseases medicine.disease_cause Sensitivity and Specificity Bacterial genetics Microbiology Feces Hemolysin Proteins fluids and secretions Bacterial Proteins Cholera Multiplex polymerase chain reaction medicine Humans Vibrio cholerae Gene Regulator gene Medicine(all) Strain (chemistry) General Medicine Multiplex PCR bacterial infections and mycoses medicine.disease Repressor Proteins Genes Bacterial bacteria Multiplex Polymerase Chain Reaction |
| Zdroj: | Asian Pacific Journal of Tropical Medicine. 6:115-118 |
| ISSN: | 1995-7645 |
| DOI: | 10.1016/s1995-7645(13)60005-x |
| Popis: | Objective To study virulence and regulatory genes ( hly A, ctx B, tcp I) in clinical strains of Vibrio cholerae ( V. cholerae ), simultaneously. Methods Three important genes, tcp I, hly A and ctx B were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method. Results According to the results of the PCR, the incidence of hly A, tcp I, and ctx B genes in clinical isolates was obtained as 94.7% (72 sample), 90.8% (69 sample), and 92.1% (70 sample), respectively. Five strains possessed all genes except ctx B, six strains possessed all genes except tcp I, four strains possessed all genes except hly A, one strain possessed only hly A and 60 strains contained a combination of three genes, Including hly A, ctx B and tcp I. Conclusions Result show that this method could be reliable to detect toxigenic-pathogenic strains of V. cholerae in Iran. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|