Occurrence of Class 1 and 2 Integrons in Resistant Enterobacteriaceae Collected from a Urban Wastewater Treatment Plant: First Report from Central Italy
| Autor: | Pierangelo Bellio, Giuseppe Celenza, Domenico Setacci, Mariagrazia Perilli, Bernardetta Segatore, Gianfranco Amicosante, Cristina Pellegrini |
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| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Microbiology (medical) fluorescein Molecular Sequence Data Immunology Microbial Sensitivity Tests Biology Medical Waste Polymerase Chain Reaction Microbiology beta-Lactam Resistance DNA sequencing Integrons law.invention Hospitals Urban Enterobacteriaceae law Humans Water Pollutants Polymerase chain reaction beta lactam antibiotic Pharmacology Base Sequence Blot hybridization Enterobacteriaceae Infections Sequence Analysis DNA biochemical phenomena metabolism and nutrition biology.organism_classification Anti-Bacterial Agents Bacterial Typing Techniques bacterial DNA Italy Sewage treatment |
| Zdroj: | Microbial Drug Resistance. 17:229-234 |
| ISSN: | 1931-8448 1076-6294 2005-2006 |
| DOI: | 10.1089/mdr.2010.0117 |
| Popis: | The purpose of this study was to investigate the presence and distribution of integron-carrying isolates among Enterobacteriaceae resistant to β-lactam antibiotics collected from a wastewater effluent of the city of L'Aquila (Italy). A total of 471 Enterobacteriaceae were collected during a period of 2 years (2005-2006). The presence and distribution of class 1 and 2 integrons was investigated by colony blot hybridization using specific probes labelled with dUTP-fluoresceine kit. The variable region of class 1 and 2 integrons was analyzed by polymerase chain reaction and DNA sequencing in 24 isolates with different random amplified polymorphic DNA profile. The characterization of class 1 and 2 integrons gene cassettes of 24 nonrelated strains showed the presence of four different arrays: dfr17-aadA5; aadA10; dfr1-sat1; dfr1-sat1-aadA1. This is the first report from Italy in which the authors confirm the presence of Enterobacteriaceae carrying class 1 and 2 integrons in a wastewater treatment plant that collects the urban and hospital discharges. |
| Databáze: | OpenAIRE |
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