Liposuction Aspirate Fluid Adipose-Derived Stem Cell Injection and Secondary Healing in Fingertip Injury
| Autor: | Marco Toscani, Cristina Spalvieri, Donata Casella, Wanda Lattanzi, Diego Ribuffo, Pasquale Fino, Giuseppe Di Taranto, Mauro Tarallo |
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| Rok vydání: | 2018 |
| Předmět: |
liposuction aspirate fluid
Male 0301 basic medicine medicine.medical_specialty medicine.medical_treatment Treatment outcome Subcutaneous Fat Aftercare Adipose tissue Pilot Projects Injections Intralesional Conservative Treatment Mesenchymal Stem Cell Transplantation 03 medical and health sciences Cell injection Amputation Traumatic Lipectomy Finger Injuries Humans Medicine Secondary healing adipose-derived stem cell Wound Healing Surgical approach business.industry fingertip injury Mesenchymal Stem Cells Middle Aged Surgery Conservative treatment Treatment Outcome 030104 developmental biology Amputation Liposuction Female business |
| Zdroj: | Plastic and Reconstructive Surgery. 142:136-147 |
| ISSN: | 0032-1052 |
| Popis: | Although fingertip injuries account for a high proportion of trauma patients, the correct surgical approach is still debated. The authors compared the traditional conservative approach and a new treatment based on the injection of liposuction aspirate fluid.Forty consecutive patients with a fingertip injury were dichotomized into group A (control group; conservative approach) and group B (treatment group). Group B underwent liposuction, followed by filtration of the lipoaspirate in a closed device (MyStem EVO kit), allowing the nonenzymatic separation of liposuction aspirate fluid, which was then injected at the site of injury. Objective outcomes were time for healing, strength, mobility of joint, and touch and sensory function. Subjective outcomes were cold intolerance, pain, hand disability, and aesthetic result. An aliquot of liposuction aspirate fluid was sent to the laboratory for cellular isolation and analysis by flow cytometry and in vitro differentiation assays.The average healing time was 22.3 days in group B and 24.9 days in group A (p0.05). Eighty-five percent of group B patients and 67 percent of group A patients scored normal to diminished superficial sensibility (p0.05). Group A had higher pain and cold intolerance scores (p0.05). Group B scored greater aesthetic and disabilities outcome results (p0.05). The cell isolation yield was 8.3 × 10(5)/ml, with a percentage of viable cells of 74.3 percent. Flow cytometry identified a mesenchymal immunophenotype, and in vitro osteogenic and adipogenic induction confirmed the bilinear potential of the isolated cells.This clinical study demonstrates for the first time the regenerative potential of liposuction aspirate fluid adipose-derived stem cells in a clinical application.Therapeutic, II. |
| Databáze: | OpenAIRE |
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