Organic matrix morphology and distribution in the palisade layer of eggshells sampled at selected periods during lay
| Autor: | M M Bain, S E Solomon, A C Fraser |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Time Factors
Morphology (linguistics) Oviposition Vesicle Mineralogy General Medicine Matrix (biology) Palisade cell Egg Shell Microscopy Electron chemistry.chemical_compound chemistry Chemical engineering Transmission electron microscopy Microscopy Animals Female Animal Science and Zoology Eggshell Paraformaldehyde Chickens Food Science |
| Zdroj: | British Poultry Science. 39:225-228 |
| ISSN: | 1466-1799 0007-1668 |
| DOI: | 10.1080/00071669889169 |
| Popis: | 1. 1 cm2 pieces of eggshells from a commercial battery flock were plasma etched to remove the outer shell membranes. 2. They were decalcified using EDTA (200 g/l, pH 6.9 to 7.0) in paraformaldehyde (20 g/l) and 25% gluteraldehyde (20 ml in 0.98 l) in phosphate buffer, then prepared for light and transmission electron microscopy. 3. Light microscopy revealed a differential distribution of matrix material within all 3 regions of the palisade layer at the beginning of lay. 4. Transmission electron microscopy revealed a more even distribution of matrix at the beginning of lay, although morphological differences were observed. At the end of lay all 3 regions showed an increase in % matrix and vesicles/10 cm2 of micrograph compared to the middle and beginning of lay periods. 5. It is hypothesised that matrix vesicles are involved in the regulation of the physiochemical environment within the forming eggshell and that the decline in shell quality associated with the end of lay is related to a concomitant change in matrix quality. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|