Transfection of Malaria Parasites
| Autor: | Chris J. Janse, M. R. van Dijk, Alan W. Thomas, Andrew P. Waters |
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| Rok vydání: | 1997 |
| Předmět: |
Plasmodium
Plasmodium berghei Transgene Genetic Vectors Plasmodium falciparum Regulatory Sequences Nucleic Acid Transfection Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology Transformation Genetic Genes Reporter parasitic diseases medicine Animals Humans Plasmodium knowlesi Molecular Biology Selectable marker Genetics biology Thymidylate Synthase DNA Protozoan medicine.disease biology.organism_classification Recombinant Proteins Malaria Tetrahydrofolate Dehydrogenase Mutagenesis Human parasite |
| Zdroj: | Methods. 13:134-147 |
| ISSN: | 1046-2023 |
| DOI: | 10.1006/meth.1997.0506 |
| Popis: | The stable genetic transformation of three phylogenetically diverse species of Plasmodium, the parasitic etiological agent of malaria, is now possible. The parasite is haploid throughout the vast majority of its life cycle. Therefore with the single selectable marker activity and protocols currently available, it is possible not only to express introduced transgenes but also to study the effects of site-specific homologous recombination such as gene knockout. Transgene expression will allow the detailed study of many aspects of the cellular biology of malaria parasites, for example, the mechanisms underlying drug resistance and protein trafficking. We describe here the methods for propagation of the two animal models (Plasmodium berghei and Plasmodium knowlesi) and for transfection of these two species and the human parasite, Plasmodium falciparum. Examples of transgene expression are given. |
| Databáze: | OpenAIRE |
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