Detection and genotyping of bovine viral diarrhea virus found contaminating commercial veterinary vaccines, cell lines, and fetal bovine serum lots originating in Mexico
| Autor: | Ninnet Gómez-Romero, Julia F. Ridpath, Antonio Verdugo-Rodríguez, Francisco Javier Basurto-Alcantara, Lauro Velazquez-Salinas |
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| Rok vydání: | 2021 |
| Předmět: |
Veterinary medicine
medicine.medical_specialty Genotype Hemorrhagic Syndrome Bovine viruses Biology Virus Cell Line 03 medical and health sciences Medical microbiology Virology medicine Animals Diarrhea Virus 2 Bovine Viral Mexico Genotyping Phylogeny 030304 developmental biology 0303 health sciences 030306 microbiology Brief Report Diarrhea Virus 1 Bovine Viral RNA Viral Vaccines General Medicine Reverse transcription polymerase chain reaction Diarrhea RNA Viral Bovine Virus Diarrhea-Mucosal Disease Cattle medicine.symptom Fetal bovine serum |
| Zdroj: | Archives of Virology |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | In this communication, we report the presence of RNA of bovine viral diarrhea virus (BVDV) as a contaminant of different biological products used in Mexico for veterinary vaccine production. For this purpose, six batches of monovalent vaccines, eight cell line batches used for vaccine production, and 10 fetal bovine serum lots (FBS) commercially available in Mexico from different suppliers were tested by reverse transcription polymerase chain reaction (RT-PCR). Viral RNA was detected in 62.5% of the samples analyzed. Phylogenetic analysis revealed the presence of the subgenotypes BVDV-1a, 1b, and BVDV-2a in the tested samples. Collectively, these findings indicate that contamination by BVDV RNA occurs in commercial vaccines and reagents used in research and production of biological products. The ramifications of this contamination are discussed. |
| Databáze: | OpenAIRE |
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