Evidence of Recombination between 3′ and 5′ LTRs in Macaques Inoculated with SIV DNA
| Autor: | C. Jane Dale, Damian F. J. Purcell, Scott Preiss, Stephen J. Kent |
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| Rok vydání: | 2002 |
| Předmět: |
Sequence analysis
animal diseases viruses Molecular Sequence Data Immunology medicine.disease_cause Virus chemistry.chemical_compound Virology medicine Animals Amino Acid Sequence Peptide sequence Gene Recombination Genetic Sequence Homology Amino Acid biology Terminal Repeat Sequences virus diseases Simian immunodeficiency virus biology.organism_classification Genes nef Infectious Diseases chemistry DNA Viral Lentivirus Simian Immunodeficiency Virus Macaca nemestrina Homologous recombination DNA |
| Zdroj: | AIDS Research and Human Retroviruses. 18:227-230 |
| ISSN: | 1931-8405 0889-2229 |
| Popis: | Proviral SIV DNA inoculation of macaques is an efficient method to initiate wild-type and attenuated SIV infections. However, we found that macaques inoculated with SIV DNA engineered to contain a single 105-bp deletion in the 3' nef/LTR overlap region had SIV sequences subsequently isolated that had partially or fully repaired the deletion with wild-type sequence. Animals inoculated with SIV DNA containing identical deletions in both the 5' and 3' LTRs did not repair the deletion. Recombination events occurred early, most likely by homologous recombination with sequences from the wild-type 5' LTR. This sequence analysis is the first demonstration of homologous recombination in vivo following administration of a single SIV strain. |
| Databáze: | OpenAIRE |
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