Purification of Pierisin, an Inducer of Apoptosis in Human Gastric Carcinoma Cells, from Cabbage Butterfly, Pieris rapae
| Autor: | Takuo Kono, Takashi Sugimura, Keiji Wakabayashi, Kotaro Koyama, Masahiko Watanabe |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cancer Research
Time Factors Cell Apoptosis Pieris rapae DNA Fragmentation Article Pierisin Stomach Neoplasms Tumor Cells Cultured medicine Animals Humans Cytotoxic T cell Inducer Key words Fragmentation (cell biology) Ammonium sulfate precipitation ADP Ribose Transferases Butterfly Dose-Response Relationship Drug biology biology.organism_classification Molecular biology medicine.anatomical_structure Oncology Immunology Insect Proteins DNA fragmentation Electrophoresis Polyacrylamide Gel Human carcinoma cells Butterflies |
| Zdroj: | Japanese Journal of Cancer Research : Gann |
| ISSN: | 0910-5050 |
| Popis: | A substance strongly cytotoxic to human carcinoma cell line TMK-1 has been found in pupae, larvae and adults of the cabbage butterfly, Pieris rapae, and named pierisin. Pierisin was purified from the pupae of P. rapae by ammonium sulfate precipitation followed by DEAE-cellulose, Phenyl-Sepharose and hydroxyapatite column chromatographies. The molecular weight of the purified pierisin, which was homogenous on SDS-polyacrylamide gel, was analyzed by mass spectrometry and found to be 98 kDa. Pierisin showed a strong cytotoxic effect, with and IC50 of 0.75 ng/ml for human gastric carcinoma TMK-1 cells. The dying cells exhibited characteristic morphological features of apoptosis, such as cell shrinkage, chromatin condensation and nuclear fragmentation. Oligonucleosomal DNA fragmentation was also observed in DNA isolated from pierisin-treated cells. Moreover, similar characteristic changes showing apoptotic cell death were observed in TMK-1 cells treated with a crude extract of pupae of P. rapae. These results indicate that pierisin from the pupae of P. rapae induces apoptosis in human carcinoma cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text