The trypanosome VSG expression site encodes adenylate cyclase and a leucine-rich putative regulatory gene
| Autor: | Isabelle Florent, M K Gross, H. Eisen, D R Storm, D T Ross, S Sather, A Raibaud |
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| Rok vydání: | 1991 |
| Předmět: |
Trypanosoma
Genes Fungal Molecular Sequence Data Restriction Mapping Adenylate kinase Saccharomyces cerevisiae Biology Cyclase Gene Expression Regulation Enzymologic General Biochemistry Genetics and Molecular Biology Open Reading Frames Leucine Sequence Homology Nucleic Acid Genes Regulator Animals heterocyclic compounds Amino Acid Sequence Molecular Biology Gene Regulator gene Genetics Base Sequence General Immunology and Microbiology General Neuroscience Blotting Northern biology.organism_classification Open reading frame Mutation Schizosaccharomyces pombe Sequence motif Sequence Alignment Cyclase activity RNA Protozoan Variant Surface Glycoproteins Trypanosoma Research Article Adenylyl Cyclases Plasmids |
| Zdroj: | The EMBO Journal. 10:2047-2053 |
| ISSN: | 0261-4189 |
| DOI: | 10.1002/j.1460-2075.1991.tb07735.x |
| Popis: | African trypanosomes are protozoan parasites that evade the host immune system by varying their dense antigenic coat. The Variant Surface Glycoprotein (VSG) is expressed exclusively from telomere-linked expression sites that contain in addition to the VSG gene, a number of open reading frames termed Expression Site Associated Genes (ESAGs). Here we demonstrate by complementation of a yeast mutant deleted for adenylate cyclase (cyr-1), that an ESAG from Trypanosoma equiperdum encodes an adenylate cyclase. Furthermore, we report that adjacent to adenylate cyclase in the expression site, is a separate open reading frame that encodes a protein sequence motif similar to the leucine-rich repeat regulatory domain of Saccharomyces cerevisiae and Schizosaccharomyces pombe adenylate cyclases. The finding of two adjacent open reading frames homologous to a single enzyme in yeast suggests that the two expression site encoded proteins may interact to regulate adenylate cyclase activity during the course of an infection. |
| Databáze: | OpenAIRE |
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