The distribution of l -asparagine synthetase in the principal organs of several mammalian and avian species
| Autor: | David A. Cooney, Harry A. Milman |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Male
Swine Kidney Biochemistry Ligases Mice Testis Carbon Radioisotopes Asparagine Amino Acids Lung chemistry.chemical_classification Leukemia CATS Temperature Brain Haplorhini Amino acid medicine.anatomical_structure Liver Female Rabbits Pancreas Turkeys Guinea Pigs Biology Guinea pig Dogs medicine Animals Asparaginase Humans Distribution (pharmacology) Thermolabile Molecular Biology Autoanalysis Sheep Ovary Biosynthesis and Degradation Neoplasms Experimental Cell Biology biology.organism_classification Rats chemistry Cats Cattle Chickens Spleen |
| Zdroj: | Biochemical Journal. 142:27-35 |
| ISSN: | 0264-6021 |
| DOI: | 10.1042/bj1420027 |
| Popis: | A survey was conducted of the distribution of l-asparagine synthetase and of l-asparaginase in the principal organs of representative mammals and birds. Although a radiometric assay was used as a routine, several additional criteria, including enzymic and chromatographic ones, were used to verify that the product of the synthetase was l-asparagine. Recoveries of exogenous l-asparagine were assessed in the presence of a number of mouse organs and found to be about 85%. In addition, evidence is presented for the existence in mouse liver of a thermolabile activity capable of destroying l-asparagine and stimulated by high concentrations of NH4+ ions. Of the organs surveyed, pancreas was generally found to synthesize l-asparagine at the most rapid rate, whereas extracts of liver catalysed the decomposition of this amide at the greatest velocity. Of the species studied, guinea pig had the highest activities of pancreatic l-asparagine synthetase and also of hepatic l-asparaginase. The pancreas of mouse and ox also were good sources of l-asparagine synthetase. |
| Databáze: | OpenAIRE |
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