Genetic analysis of an Erysipelothrix rhusiopathiae swine isolate determined to be serovar 2 by a gel double diffusion test but serovar 1a/2 by a serotyping PCR assay
| Autor: | Kazumasa Shiraiwa, Yohsuke Ogawa, Momoko Nakayama, Yoshihiro Shimoji, Masahiro Eguchi, Sayaka Nishikawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Serotype
040301 veterinary sciences Swine serovar 2 Pcr assay Biology Erysipelothrix rhusiopathiae Serogroup Genetic analysis Microbiology 0403 veterinary science 03 medical and health sciences Erysipelothrix Infections Multiplex polymerase chain reaction Animals Genetic Testing Serotyping Gene 030304 developmental biology Swine Diseases 0303 health sciences General Veterinary Strain (chemistry) ERH_1440 Bacteriology 04 agricultural and veterinary sciences biology.organism_classification Note Chromosomal region Erysipelothrix serovar 1a |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | We previously developed a multiplex PCR assay for the differentiation of serovar 1a, 1b, 2 and 5 strains of Erysipelothrix rhusiopathiae. In this study, we analyzed the serovar-defining chromosomal region of a serovar 2 swine isolate, which was PCR-positive for both serovars 1a and 2 by the multiplex PCR assay. Genetic analysis of the chromosomal region revealed that, as in serovar 1a strains, the ERH_1440 gene, which is usually truncated or missing in serovar 2 strains, was intact in this strain. This paper first shows an E. rhusiopathiae serovar 2 strain possessing an intact ERH_1440 gene and suggests that care may be needed when determining the serovar of such rare strains by PCR assay. |
| Databáze: | OpenAIRE |
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