Enumeration of Clostridium perfringens spores in groundwater samples: comparison of six culture media
| Autor: | M. Araujo, M. J. Gómez, Rosa Ana Sueiro, M. J. Garrido |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
food.ingredient Clostridium perfringens Colony Count Microbial Filtration technique Biology medicine.disease_cause Microbiology food Enumeration medicine Agar media_common.cataloged_instance Food science European union Molecular Biology Soil Microbiology media_common Spores Bacterial Culture Media Spore Colony count Water Microbiology Soil microbiology |
| Zdroj: | Journal of Microbiological Methods. 57:175-180 |
| ISSN: | 0167-7012 |
| DOI: | 10.1016/j.mimet.2003.12.010 |
| Popis: | In order to investigate the ability of Fluorocult-supplemented TSC agar (TSCF (Fluorocult supplemented TSC-agar): prepared from Tryptose Sulfite Cycloserine Agar Base (Merck), D-cycloserine (Fluka Chemika, USA), and fluorocult TSC-Agar supplement (Merck)) for detecting spores of Clostridium perfringens in water, we analyzed groundwater samples, pretreated by heating to 80 degrees C/5 min, using this fluorogenic medium together with five other media: mCP agar (Panreac; Cultimed), TSC agar (Merck, Germany), TSN agar (Merck), and SPS agar (BBL, USA) by the membrane filtration technique, and Wilson-Blair agar (WB) following the still-in-force Spanish official method. Variance analysis of the data obtained shows statistically significant differences in the counts obtained between media employed in this work. The C. perfringens spore counts on mCP agar were significantly lower (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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