QTL and candidate gene mapping for polyphenolic composition in apple fruit

Autor: Rebecca A Henry, Andrew C. Allan, Michela Troggio, Susan E. Gardiner, Christelle M. Andre, Jenny Fraser, William A. Laing, Tony K. McGhie, Maysoon Rassam, David Chagné, Célia Krieger, Massimo Pindo, Michael B. Sullivan
Přispěvatelé: Plant & Food Research, Laboratoire Agronomie et Environnement (LAE), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Mt Albert Res Ctr, DSIR, University of Otago [Dunedin, Nouvelle-Zélande], Centro Ricerca e Innovazione, Fondazione Edmund Mach, Instituto Agrario S. Michele all' Adige, Sch Biol Sci, University of Auckland [Auckland], Pipfruit Internal Investment Programme (PIIP), New Zealand Institute for Plant & Food Research Limited
Jazyk: angličtina
Rok vydání: 2012
Předmět:
0106 biological sciences
QTL mapping
[SDV]Life Sciences [q-bio]
NEW-ZEALAND
RAPD MARKERS
Malus x domestica
Plant Science
01 natural sciences
Antioxidants
anthocyanin
tannin
chemistry.chemical_compound
Flavonols
lcsh:Botany
ANTIOXIDANT
Food science
TRANSCRIPTION FACTOR
2. Zero hunger
chemistry.chemical_classification
0303 health sciences
education.field_of_study
Phenylpropanoid
biology
polyphenolic
candidate gene
flavonoid
flavanol
metabolomics
MALUS X DOMESTICA
CHLOROGENIC ACID
LINKAGE MAPS
RED APPLES
IDENTIFICATION
BIOSYNTHESIS
Chromosome Mapping
Leucoanthocyanidin reductase
food and beverages
Marker-assisted selection
lcsh:QK1-989
Settore AGR/07 - GENETICA AGRARIA
Phenotype
Malus
[SDE]Environmental Sciences
Flavonol
Genome
Plant
Research Article
DNA
Plant
Quantitative Trait Loci
Population
Quantitative trait locus
Polymorphism
Single Nucleotide
03 medical and health sciences
Botany
education
030304 developmental biology
Polyphenols
biology.organism_classification
chemistry
Fruit
Anthocyanin
010606 plant biology & botany
Zdroj: BMC Plant Biology (12), . (2012)
BMC Plant Biology
BMC Plant Biology, BioMed Central, 2012, 12, ⟨10.1186/1471-2229-12-12⟩
ResearcherID
BMC Plant Biology, Vol 12, Iss 1, p 12 (2012)
ISSN: 1471-2229
Popis: Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; Background: The polyphenolic products of the phenylpropanoid pathway, including proanthocyanidins, anthocyanins and flavonols, possess antioxidant properties that may provide health benefits. To investigate the genetic architecture of control of their biosynthesis in apple fruit, various polyphenolic compounds were quantified in progeny from a 'Royal Gala'x 'Braeburn' apple population segregating for antioxidant content, using ultra high performance liquid chromatography of extracts derived from fruit cortex and skin. Results: Construction of genetic maps for 'Royal Gala' and 'Braeburn' enabled detection of 79 quantitative trait loci (QTL) for content of 17 fruit polyphenolic compounds. Seven QTL clusters were stable across two years of harvest and included QTLs for content of flavanols, flavonols, anthocyanins and hydroxycinnamic acids. Alignment of the parental genetic maps with the apple whole genome sequence in silico enabled screening for co-segregation with the QTLs of a range of candidate genes coding for enzymes in the polyphenolic biosynthetic pathway. This co-location was confirmed by genetic mapping of markers derived from the gene sequences. Leucoanthocyanidin reductase (LAR1) co-located with a QTL cluster for the fruit flavanols catechin, epicatechin, procyanidin dimer and five unknown procyanidin oligomers identified near the top of linkage group (LG) 16, while hydroxy cinnamate/quinate transferase (HCT/HQT) co-located with a QTL for chlorogenic acid concentration mapping near the bottom of LG 17. Conclusion: We conclude that LAR1 and HCT/HQT are likely to influence the concentration of these compounds in apple fruit and provide useful allele-specific markers for marker assisted selection of trees bearing fruit with healthy attributes.
Databáze: OpenAIRE
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