Rotavirus-induced fusion from without in tissue culture cells
| Autor: | M M Falconer, Joanna M. Gilbert, A M Roper, Harry B. Greenberg, J S Gavora |
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| Rok vydání: | 1995 |
| Předmět: |
Rotavirus
medicine.drug_class Viral protein viruses Immunology Biology Monoclonal antibody medicine.disease_cause Kidney Microbiology Virus Cell Line Cell Fusion fluids and secretions Capsid Species Specificity Virology Chlorocebus aethiops medicine Animals Edetic Acid Cell fusion Cell Membrane virus diseases Antibodies Monoclonal Hydrogen-Ion Concentration Macaca mulatta Kinetics Microscopy Electron Cholesterol Cell culture Insect Science Capsid Proteins Cattle Fusion mechanism Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0022-538X |
| Popis: | We present the first evidence of fusion from without induced in tissue culture cells by a nonenveloped virus. Electron micrographs of two strains of rotavirus, bovine rotavirus C486 and rhesus rotavirus, show that virally mediated cell-cell fusion occurs within 1 h postinfection. Trypsin activation is necessary for rotavirus to mediate cell-cell fusion. The extent of fusion is relative to the amount of virus used, and maximum fusion occurs between pHs 6.5 and 7.5. Fusion does not require virus-induced protein synthesis, as virus from both an empty capsid preparation and from an EDTA-treated preparation, which is noninfectious, can induce fusion. Incubation of rotavirus with neutralizing and nonneutralizing monoclonal antibodies before addition to cells indicates that viral protein 4 (VP4; in the form of VP5* and VP8*) and VP7 are involved in fusion. Light and electron micrographs document this fusion, including the formation of pores or channels between adjacent fused cells. These data support direct membrane penetration as a possible route of infection. Moreover, the assay should be useful in determining the mechanisms of cell entry by rotavirus. |
| Databáze: | OpenAIRE |
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