In vitro assays for cobblestone area-forming cells, LTC-IC, and CFU-C

Autor:	van Os, Ronald P, Dethmers-Ausema, Bertien, de Haan, Gerald, Bunting, Kevin
Přispěvatelé:	Damage and Repair in Cancer Development and Cancer Treatment (DARE), Stem Cell Aging Leukemia and Lymphoma (SALL)
Jazyk:	angličtina
Rok vydání:	2008
Předmět:	education.field_of_study Population Biology Stem cell marker Hematopoietic Stem Cells Molecular biology Cell biology Cell Line Endothelial stem cell Haematopoiesis In vivo Humans Cell Lineage Stem cell Progenitor cell Stromal Cells education Cobblestone Area-Forming Cells
Zdroj:	Methods in Molecular Biology ISBN: 9781588298683 Methods in Molecular Biology: Hematopoietic Stem Cell Protocols, 430(III), 143-57
DOI:	10.1007/978-1-59745-182-6_10
Popis:	Various assays exist that measure the function of hematopoietic stemcells (HSCs). In this chapter, in vitro assays are described that measure the frequency of progenitors (colony-forming unit in culture; CFU-C), stem cells (long-term culture-initiating cell; LTC-IC), or both (cobblestone area-forming cell assay; CAFC). These assays measure the potential of a test cell population retrospectively, i.e., at the time its activity is evident when the stem cell itself is often not detectable anymore. Although the in vitro LTC-IC and CAFC assays have been shown to correlate with in vivo activity, in vivo transplantation assays, where it can be shown that cells possess the ability to indefinitely repopulate all blood lineages, are the ultimate proof for HSC activity. Nevertheless, these in vitro assays provide an excellent method to screen for stem cell activity of a putative stem cell population or for screening the effect of a certain treatment on HSCs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff18e650efe83835de74c2cb4561be86 https://doi.org/10.1007/978-1-59745-182-6_10 Zobrazit plný text záznamu