A fluorescent multiplex-DGGE screening test for mutations in the BRCA1 gene
| Autor: | Graciela Kuperstein, Elaine Jack, Steven A. Narod |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
RNA Splicing
DNA Mutational Analysis Locus (genetics) Biology medicine.disease_cause Polymerase Chain Reaction Exon Multiplex polymerase chain reaction medicine Humans Multiplex Genetic Testing Gene Genetics (clinical) Genetics Ovarian Neoplasms Mutation Polymorphism Genetic BRCA1 Protein Exons Amplicon Molecular biology Electrophoresis Polyacrylamide Gel Female Temperature gradient gel electrophoresis |
| Zdroj: | Genetic testing. 10(1) |
| ISSN: | 1090-6576 |
| Popis: | Screening for mutations in the BRCA1 gene is challenging because of the wide spectrum of mutations found in this large gene. As the extensive exon 11 is commonly screened by the protein truncation test (PTT), here a fluorescent multiplex denaturing gradient gel electrophoresis (FMD) mutation screening technique was developed to test the remaining numerous small exons and splice sites of the gene. The method is based upon the use of an efficient multiplex polymerase chain reaction (PCR) amplification of the target regions, followed by denaturing gradient gel electrophoresis (DGGE) separation of the amplicon mixture, and the immediate achievement of results by wet gel scanning. The technique was applied to screen 16 samples with different BRCA1 sequence variants distributed over 12 exons. All variants were detected. In addition, 188 DNA samples from ovarian cancer patients were screened, identifying 22 new sequence variants (11.7% of the samples) and 243 common polymorphisms in the BRCA1 locus. Variants included 16 single nucleotide substitutions, 3 deletions of 2 nucleotides, 1 deletion of 4 nucleotides, and 2 insertions of 1 nucleotide. The FMD test provides an accurate, fast, nonradioactive and cost-efficient way to scan the BRCA1 gene with high sensitivity and an ease of result interpretation. This technique may prove to be a useful research tool for the detection of mutations and polymorphisms in the BRCA1 gene and for large-scale epidemiologic studies. |
| Databáze: | OpenAIRE |
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