Membrane Proteomic Profiling of Soybean Leaf and Root Tissues Uncovers Salt-Stress-Responsive Membrane Proteins

Autor:	Hafiz Mamoon Rehman, Shengjie Chen, Shoudong Zhang, Memoona Khalid, Muhammad Uzair, Phillip A. Wilmarth, Shakeel Ahmad, Hon-Ming Lam
Rok vydání:	2022
Předmět:	Proteomics salt stress membrane proteome soybean orbitrap stress-inducible proteins label-free quantification mass spectrometry Organic Chemistry Membrane Proteins General Medicine Sodium Chloride Plant Roots Salt Stress Catalysis Computer Science Applications Plant Leaves Inorganic Chemistry Seedlings Stress Physiological Soybeans Physical and Theoretical Chemistry Molecular Biology Spectroscopy Plant Proteins
Zdroj:	International Journal of Molecular Sciences; Volume 23; Issue 21; Pages: 13270
ISSN:	1422-0067
DOI:	10.3390/ijms232113270
Popis:	Cultivated soybean (Glycine max (L.)), the world’s most important legume crop, has high-to-moderate salt sensitivity. Being the frontier for sensing and controlling solute transport, membrane proteins could be involved in cell signaling, osmoregulation, and stress-sensing mechanisms, but their roles in abiotic stresses are still largely unknown. By analyzing salt-induced membrane proteomic changes in the roots and leaves of salt-sensitive soybean cultivar (C08) seedlings germinated under NaCl, we detected 972 membrane proteins, with those present in both leaves and roots annotated as receptor kinases, calcium-sensing proteins, abscisic acid receptors, cation and anion channel proteins, proton pumps, amide and peptide transporters, and vesicle transport-related proteins etc. Endocytosis, linoleic acid metabolism, and fatty acid biosynthesis pathway-related proteins were enriched in roots whereas phagosome, spliceosome and soluble NSF attachment protein receptor (SNARE) interaction-related proteins were enriched in leaves. Using label-free quantitation, 129 differentially expressed membrane proteins were found in both tissues upon NaCl treatment. Additionally, the 140 NaCl-induced proteins identified in roots and 57 in leaves are vesicle-, mitochondrial-, and chloroplast-associated membrane proteins and those with functions related to ion transport, protein transport, ATP hydrolysis, protein folding, and receptor kinases, etc. Our proteomic results were verified against corresponding gene expression patterns from published C08 RNA-seq data, demonstrating the importance of solute transport and sensing in salt stress responses.
Databáze:	OpenAIRE
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