Balancing Hedgehog, a retention and release equilibrium given by Dally, Ihog, Boi and shifted/DmWif
| Autor: | Ainhoa Callejo, Emanuela Mollica, Isabel Guerrero, M. Carmen Rodríguez-Navas, Eléanor Simon, Aphrodite Bilioni, Ana-Citlali Gradilla, David Sánchez-Hernández, Carmen Ibañez |
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| Rok vydání: | 2013 |
| Předmět: |
Hedgehog gradient
Glypican Genotype Green Fluorescent Proteins Model system Receptors Cell Surface Dally Biology Models Biological 03 medical and health sciences Ihog Shifted/DmWif Boi Animals Drosophila Proteins Hedgehog Proteins Transgenes Hedgehog Molecular Biology 030304 developmental biology 0303 health sciences Membrane Glycoproteins Models Genetic 030302 biochemistry & molecular biology Cell Membrane Anatomy Cell Biology Transmembrane protein Cell biology Protein Structure Tertiary Imaginal disc Drosophila melanogaster Gene Expression Regulation Microscopy Fluorescence Intercellular Signaling Peptides and Proteins Proteoglycans Carrier Proteins Cytoneme Morphogen Developmental Biology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2012.12.013 |
| Popis: | Hedgehog can signal both at a short and long-range, and acts as a morphogen during development in various systems. We studied the mechanisms of Hh release and spread using the Drosophila wing imaginal disc as a model system for polarized epithelium. We analyzed the cooperative role of the glypican Dally, the extracellular factor Shifted (Shf, also known as DmWif), and the Immunoglobulin-like (Ig-like) and Fibronectin III (FNNIII) domain-containing transmembrane proteins, Interference hedgehog (Ihog) and its related protein Brother of Ihog (Boi), in the stability, release and spread of Hh. We show that Dally and Boi are required to prevent apical dispersion of Hh; they also aid Hh recycling for its release along the basolateral part of the epithelium to form a long-range gradient. Shf/DmWif on the other hand facilitates Hh movement restrained by Ihog, Boi and Dally, establishing equilibrium between membrane attachment and release of Hh. Furthermore, this protein complex is part of thin filopodia-like structures or cytonemes, suggesting that the interaction between Dally, Ihog, Boi and Shf/DmWif is required for cytoneme-mediated Hh distribution during gradient formation. © 2013 Elsevier Inc. BFU2008-03320/BMC,BFU2011-25987 from Consolider ProgramCDS2007-00008 from theSpanishMICINN; MarieCurieRTNFP6 and FP7; Fundacion Ramón Areces; JAE-CSICprogram; Spanish MICINN |
| Databáze: | OpenAIRE |
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