Purification and Initial Characterization of Ara h 7, a Peanut Allergen from the 2S Albumin Protein Family
| Autor: | Govardus A.H. de Jong, Danijela Apostolovic, Adrie H. Westphal, Harmen H.J. de Jongh, Phil Johnson, Justin T. Marsh, Steve L. Taylor, Stef J. Koppelman, Joseph L. Baumert |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Gene isoform Arachis Protein family Biochemie medicine.disease_cause Immunoglobulin E Cleavage (embryo) 01 natural sciences Biochemistry Ara h 7 Hydroxyproline chemistry.chemical_compound Allergen 2S albumin Albumins Arachis hypogaea medicine Peanut Hypersensitivity heterocyclic compounds peanuts Protein secondary structure Plant Proteins biology 010401 analytical chemistry food and beverages General Chemistry Allergens Antigens Plant biochemical phenomena metabolism and nutrition 0104 chemical sciences carbohydrates (lipids) chemistry biology.protein 2s albumin lipids (amino acids peptides and proteins) General Agricultural and Biological Sciences 2S Albumins Plant 010606 plant biology & botany allergen |
| Zdroj: | Journal of Agricultural and Food Chemistry, 69(22), 6318-6329 Journal of Agricultural and Food Chemistry 69 (2021) 22 |
| ISSN: | 0021-8561 |
| Popis: | 2S albumins are important peanut allergens. Within this protein family, Ara h 2 and Ara h 6 have been described in detail, but Ara h 7 has received little attention. We now describe the first purification of Ara h 7 and its characterization. Two Ara h 7 isoforms were purified from peanuts. Mass spectrometry revealed that both the isoforms have a post-translation cleavage, a hydroxyproline modification near the N-terminus, and four disulfide bonds. The secondary structure of both Ara h 7 isoforms is highly comparable to those of Ara h 2 and Ara h 6. Both Ara h 7 isoforms bind IgE, and Ara h 7 is capable of inhibiting the binding between Ara h 2 and IgE, suggesting at least partially cross-reactive IgE epitopes. Ara h 7 was found in all main market types of peanut, at comparable levels. This suggests that Ara h 7 is a relevant allergen from the peanut 2S albumin protein family. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|