Involvement and characterization of NLRCs and pyroptosis-related genes in Nile tilapia (Oreochromis niloticus) immune response
Autor: | Qi Li, Baijian Jiang, Zhiqiang Zhang, Yongxiong Huang, Zhou Xu, Xinjin Chen, Yu Huang, Jichang Jian, Qingpi Yan |
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Rok vydání: | 2022 |
Předmět: |
Fish Proteins
Inflammasomes Cichlids General Medicine Aquatic Science Antiviral Agents Immunity Innate Anti-Bacterial Agents Streptococcus agalactiae Fish Diseases Gene Expression Regulation Caspases Streptococcal Infections NLR Family Pyrin Domain-Containing 3 Protein Pyroptosis Animals Environmental Chemistry |
Zdroj: | Fish & Shellfish Immunology. 130:602-611 |
ISSN: | 1050-4648 |
Popis: | Pyroptosis is an inflammatory and programmed cell death initiated by the formation of the inflammasome, which consists of NLR, ASC, and Caspase. Pyroptosis has received growing attention due to its association with innate immunity and various diseases. However, the involvement and induction of the NLRCs and pyroptosis-related genes in fish immunity remain poorly studied. In this study, several NLRCs and pyroptosis-related genes in Nile tilapia (Oreochromis niloticus) were identified and characterized. Their involvement in bacterial infection and expression profiles in Nile tilapia lymphocyte responses were also assessed. Overall, three NLRC members (NOD1, NOD2, and NLRC3) and five pyroptosis-related genes (ASC1, Caspase1, Gsdme, NLRP3, and NLRP14) in Nile tilapia were cloned and characterized. The transcript levels of these molecules were broadly distributed in various tissues with comparatively high expression in the gills, intestine, and spleen. Their transcripts were also induced during Streptococcus agalactiae or Aeromonas hydrophila infection. Moreover, they were primarily expressed in T cells, NCCs, and Mo/Mφ and showed antibacterial and partially antiviral responses. The present study lays a theoretical foundation for further investigation of the pyroptosis mechanisms in fish as well as the evolution of the antiviral roles of pyroptosis in vertebrates. |
Databáze: | OpenAIRE |
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