Host microRNA-203a Is antagonistic to the progression of foot-and-mouth disease virus infection
| Autor: | Teresa de los Santos, Paul Lawrence, Michael LaRocco, Joseph Gutkoska, Elizabeth Ramirez-Medina |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Picornavirus Swine animal diseases viruses Virus Replication Virus Cell Line Madin Darby Canine Kidney Cells 03 medical and health sciences Dogs In vivo Virology microRNA Animals RNA Small Interfering Host factor biology virus diseases Transfection biochemical phenomena metabolism and nutrition Viral Load biology.organism_classification MicroRNAs 030104 developmental biology Cell culture Foot-and-Mouth Disease Virus Protein Biosynthesis Disease Progression Cattle RNA Interference Foot-and-mouth disease virus Enterovirus Bovine |
| Zdroj: | Virology. 504 |
| ISSN: | 1096-0341 |
| Popis: | Sam68 was previously shown to be a critical host factor for foot-and-mouth disease virus (FMDV) replication. MicroRNA (miR) miR-203a is reportedly a negative regulator of Sam68 expression both in vitro and in vivo. Here, transfection of miR-203a-3p and miR-203a-5p mimics separately and in combination in a porcine cell line followed by FMDV infection resulted in diminished viral protein synthesis and a 4 and 6log reduction in virus titers relative to negative controls, respectively. Unexpectedly, Sam68 expression was increased by miR-203a-5p transfection, but not miR-203a-3p. miR-203a-5p also down-regulated Survivin expression, which was predicted to play a role in FMDV infection. Moreover, miR-203a-5p but not miR-203a-3p affected a reduction in FMDV viral RNA. These effects were not replicated with a related Picornavirus, suggesting FMDV specificity. Importantly, miR-203a-3p and miR-203a-5p impaired FMDV infection across multiple FMDV serotypes. We concluded that miR-203a-3p and miR-203a-5p represent attractive potential naturally occurring bio-therapeutics against FMDV. |
| Databáze: | OpenAIRE |
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