Effect of B7.1-transfected human colon cancer cells on the induction of autologous tumour-specific cytotoxic T cells
| Autor: | Kyouka Ryo, Naoaki Hayashi, Katsumi Yamauchi, Yuko Miyazono, Takaji Furukawa, Yumiko Kamogawa, Takanobu Kameoka, Maki Mitsuhashi |
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| Rok vydání: | 1999 |
| Předmět: |
CD4-Positive T-Lymphocytes
Biology CD8-Positive T-Lymphocytes Lymphocyte Activation Transfection Interleukin 21 Epitopes Lymphocytes Tumor-Infiltrating NK-92 Cytotoxic T cell Humans Antigen-presenting cell Cells Cultured Lymphokine-activated killer cell Hepatology Tumor-infiltrating lymphocytes Histocompatibility Antigens Class I Gastroenterology Middle Aged Natural killer T cell Cytotoxicity Tests Immunologic Flow Cytometry Intercellular Adhesion Molecule-1 Immunology Colonic Neoplasms Cancer research Interleukin 12 B7-1 Antigen Leukocytes Mononuclear Interleukin-2 Female Cell Division T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of gastroenterology and hepatology. 14(10) |
| ISSN: | 0815-9319 |
| Popis: | Background: The induction of tumour-specific immunity is important for advanced cancer therapy. There are many molecules, including costimulatory molecules, that have been identified as the activator for tumour-specific T cells. Methods: To induce autologous tumour-specific cytotoxic T lymphocytes (CTL) more effectively, we studied whether the expression of the B7 gene may render human colon cancer cells able to stimulate autologous peripheral blood mononuclear cells (PBMC) to become tumour-specific cytotoxic T cells. After the establishment of a B7.1 gene transfected tumour cell line, Cw2/B7.1, we first examined its stimulatory effect on autologous PBMC and subsequently, its effect on the induction of parental cell (Cw2)-specific CTL. Results: The results showed that Cw2/B7.1 had a more potent stimulatory effect on PBMC for the induction of both proliferation and cytotoxicity than Cw2. By adding a low dose of interleukin-2, Cw2/B7.1-activated killer cell activity was significantly increased. The specificity of Cw2/B7.1-activated killer cells was demonstrated by the absence of their cytotoxicity to either human lymphocyte antigen (HLA)-A33 identical (ORF) or HLA-non-identical (MT) allogenic colon cancer cell lines. Furthermore, such Cw2-specific cytotoxic activity was significantly reduced by the deletion of CD8+ cells but not CD4+ cells, indicating that these killer cells were mainly CD8+ T cells. Conclusions: Thus, our results demonstrate that, by using B7.1 gene-transfected tumour cell lines, we effectively induced autologous tumour-specific CTL. These results will provide us with new tools for adoptive immunotherapy for colon cancer patients. © 1999 Blackwell Science Asia Pty Ltd |
| Databáze: | OpenAIRE |
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