Single-strand DNA detection using a planar photonic-crystal-waveguide-based sensor

Autor:	Veronica Toccafondo, Sergio Peransi-Llopis, Ángel Maquieira, Amadeu Griol, Jaime García-Rupérez, María-José Bañuls, J. G. Castelló
Rok vydání:	2010
Předmět:	Materials science DNA Single-Stranded Physics::Optics 02 engineering and technology 01 natural sciences 010309 optics chemistry.chemical_compound Planar Optics Fiber Bragg grating Limit of Detection Complementary DNA 0103 physical sciences Surface plasmon resonance Photonic crystal Detection limit Photons Quantitative Biology::Biomolecules business.industry Nucleic Acid Hybridization Optical Devices 021001 nanoscience & nanotechnology Quantitative Biology::Genomics Atomic and Molecular Physics and Optics chemistry Microscopy Electron Scanning Optoelectronics 0210 nano-technology business Refractive index DNA
Zdroj:	Optics Letters
ISSN:	1539-4794 0146-9592
Popis:	We report an experimental demonstration of single-strand DNA (ssDNA) detection at room temperature using a photonic-crystal-waveguide-based optical sensor. The sensor surface was previously biofunctionalized with ssDNA probes to be used as specific target receptors. Our experiments showed that it is possible to detect these hybridization events using planar photonic-crystal structures, reaching an estimated detection limit as low as 19.8 nM for the detection of the complementary DNA strand.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff10cb92f16f5113ce5b99215f355fba https://doi.org/10.1364/ol.35.003673 Zobrazit plný text záznamu