Real-Time Analysis of Oxygen Gradient in Oocyte Respiration Using a High-Density Microelectrode Array
| Autor: | G. Catandi, Yusra Obeidat, Elaine M. Carnevale, William Tedjo, Thomas M. Chen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Clinical Biochemistry Microfluidics microfluidics chemistry.chemical_element Biosensing Techniques 02 engineering and technology Oxygen Article 03 medical and health sciences Animals Image resolution 030304 developmental biology 0303 health sciences Pixel microelectrode array Respiration Oxides General Medicine Multielectrode array 021001 nanoscience & nanotechnology Electrochemical gas sensor electrochemistry chemistry oxygen consumption rate Temporal resolution Oocytes CMOS biosensor Cattle Limiting oxygen concentration oxygen flux oxygen concentration gradient 0210 nano-technology Microelectrodes TP248.13-248.65 Biotechnology Biomedical engineering |
| Zdroj: | Biosensors Volume 11 Issue 8 Biosensors, Vol 11, Iss 256, p 256 (2021) |
| ISSN: | 2079-6374 |
| DOI: | 10.3390/bios11080256 |
| Popis: | Physiological events related to oxygen concentration gradients provide valuable information to determine the state of metabolizing biological cells. The existing oxygen sensing methods (i.e., optical photoluminescence, magnetic resonance, and scanning electrochemical) are well-established and optimized for existing in vitro analyses. However, such methods also present various limitations in resolution, real-time sensing performance, complexity, and costs. An electrochemical imaging system with an integrated microelectrode array (MEA) would offer attractive means of measuring oxygen consumption rate (OCR) based on the cell’s two-dimensional (2D) oxygen concentration gradient. This paper presents an application of an electrochemical sensor platform with a custom-designed complementary-metal-oxide-semiconductor (CMOS)-based microchip and its Pt-coated surface MEA. The high-density MEA provides 16,064 individual electrochemical pixels that cover a 3.6 mm × 3.6 mm area. Utilizing the three-electrode configuration, the system is capable of imaging low oxygen concentration (18.3 µM, 0.58 mg/L, or 13.8 mmHg) at 27.5 µm spatial resolution and up to 4 Hz temporal resolution. In vitro oxygen imaging experiments were performed to analyze bovine cumulus-oocytes-complexes cells OCR and oxygen flux density. The integration of a microfluidic system allows proper bio-sample handling and delivery to the MEA surface for imaging. Finally, the imaging results are processed and presented as two-dimensional (2D) heatmaps, representing the dissolved oxygen concentration in the immediate proximity of the MEA. This paper provides the results of real-time 2D imaging of OCR of live cells/tissues to gain spatial and temporal dynamics of target cell metabolism. |
| Databáze: | OpenAIRE |
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