Purification, crystallization and preliminary crystallographic analysis of porcine aldose reductase
| Autor: | Lawrence J. DeLucas, J.M. Petrash, Edwin M. Westbrook, Charles E. Bugg, Y. Sudhakar Babu, Karen M. Moore, Sthanam V.L. Narayana, T. G. Flynn, O. El-Kabbani |
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| Rok vydání: | 1991 |
| Předmět: |
Aldose reductase
Protein Conformation Swine Computer aid Polyethylene glycol Triclinic crystal system law.invention chemistry.chemical_compound Crystallography X-Ray Diffraction chemistry Aldehyde Reductase Structural Biology law X-ray crystallography Animals Molecule Crystallization Molecular Biology |
| Zdroj: | Journal of Molecular Biology. 218:695-698 |
| ISSN: | 0022-2836 |
| Popis: | Large crystals of porcine aldose reductase have been grown from polyethylene glycol solutions. The crystals are triclinic, space-group P 1, with a = 81.3 A , b = 85.9 A , c = 56.6 A , α = 102.3 °, β = 103.3 ° and ψ = 79.0 ° . The crystals grow within ten days to dimensions of 0.6 mm × 0.4 mm × 0.2 mm and diffract to at least 2.5 A. There are four molecules in the unit cell related by a set of three mutually perpendicular non-crystallographic 2-fold axes. |
| Databáze: | OpenAIRE |
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