Generation of a lentiviral vector system to efficiently express bioactive recombinant human prolactin hormones
| Autor: | Hernán Alarcón, Antonio Rodríguez, Rocío García Peinado, Jesús Vázquez, Filip Lim, Elena Bonzón-Kulichenko |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Genetic Vectors Cell Culture Techniques 030209 endocrinology & metabolism Biology Protein Engineering Biochemistry Viral vector law.invention Cell Line 03 medical and health sciences Paracrine signalling 0302 clinical medicine Endocrinology law Paracrine Communication Animals Humans Autocrine signalling Molecular Biology Hormone activity Lentivirus Wild type Prolactin Recombinant Proteins Cell biology Rats Autocrine Communication 030104 developmental biology HEK293 Cells Cell culture Recombinant DNA |
| Zdroj: | Molecular and cellular endocrinology. 499 |
| ISSN: | 1872-8057 |
| Popis: | The contribution of the pleiotropic hormone Prolactin (PRL) to several physiological and pathological processes is still unknown. To clarify the role of PRL in these processes during the last decade, different human PRL antagonists have been produced to either partially or fully block the wild type hormone activity. In this work, we have cloned these wild type and antagonist sequences in lentivectors (LV) to express them as recombinant self-processing polypeptides by employing a P2A sequence (hPRL-P2A-GFP). We show that these LVs can efficiently transduce and express the hPRL proteins in different cell types and that the P2A sequence does not affect their activities. Additionally, we have tested their activities in paracrine and autocrine cell culture experiments. Our results demonstrate that these recombinant hPRL-P2A proteins are bioactive in both paracrine and autocrine modes, highlighting the potential usefulness of these hPRL-containing LVs for determining the contribution of hPRL to different biological processes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect