Membrane interaction and perturbation mechanisms induced by two cationic cell penetrating peptides with distinct charge distribution

Autor: Isabel D. Alves, Sandrine Sagan, Nicole Goasdoué, Solange Lavielle, Gérard Chassaing, Soline Aubry, Isabelle Correia, Cécile Galanth
Přispěvatelé: Synthèse, Structure et Fonction de Molécules Bioactives (SSFMB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC), Proteines Biochimie Structurale et Fonctionnelle (FRE 2852), Peptidome de la Peau des Amphibiens (FRE 2852), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Léon Brillouin (LLB - UMR 12), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, Sorbonne Université (SU), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Erythrocytes
Cooperativity
Cell-Penetrating Peptides
01 natural sciences
Biochemistry
chemistry.chemical_compound
Cricetinae
Lipid bilayer
[PHYS]Physics [physics]
0303 health sciences
Calorimetry
Differential Scanning
[CHIM.ORGA]Chemical Sciences/Organic chemistry
Vesicle
Bilayer
Circular Dichroism
Temperature
Phosphatidylglycerols
Klebsiella pneumoniae
Membrane
Ethanolamines
Fatty Acids
Unsaturated
Thermodynamics
Dimyristoylphosphatidylcholine
Staphylococcus aureus
Biophysics
Phospholipid
CHO Cells
Microbial Sensitivity Tests
010402 general chemistry
03 medical and health sciences
Cricetulus
Animals
Humans
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Molecular Biology
Nuclear Magnetic Resonance
Biomolecular
030304 developmental biology
Phosphatidylethanolamine
Dose-Response Relationship
Drug
Cell Membrane
Phosphorus Isotopes
0104 chemical sciences
chemistry
Models
Chemical
Liposomes
Cell-penetrating peptide
Bacillus megaterium
Carrier Proteins
Peptides
Antimicrobial Cationic Peptides
Zdroj: BBA-Biochimica et Biophysica Acta
BBA-Biochimica et Biophysica Acta, Elsevier, 2010, 1780 ((7-8)), pp.948-59. ⟨10.1016/j.bbagen.2008.04.004⟩
Biochimica et Biophysica Acta-Molecular Cell Research
Biochimica et Biophysica Acta-Molecular Cell Research, Elsevier, 2008, 1780 (7-8), pp.948-59. ⟨10.1016/j.bbagen.2008.04.004⟩
Biochimica et Biophysica Acta (BBA)-General Subjects
Biochimica et Biophysica Acta (BBA)-General Subjects, Elsevier, 2008, 1780 (7-8), pp.948-959. ⟨10.1016/j.bbagen.2008.04.004⟩
BBA-Biochimica et Biophysica Acta, 2010, 1780 ((7-8)), pp.948-59. ⟨10.1016/j.bbagen.2008.04.004⟩
Biochimica et Biophysica Acta (BBA)-General Subjects, 2008, 1780 (7-8), pp.948-959. ⟨10.1016/j.bbagen.2008.04.004⟩
Biochimica et Biophysica Acta-Molecular Cell Research, 2008, 1780 (7-8), pp.948-59. ⟨10.1016/j.bbagen.2008.04.004⟩
ISSN: 0006-3002
0167-4889
0304-4165
DOI: 10.1016/j.bbagen.2008.04.004⟩
Popis: International audience; Independently from the cell penetrating peptide uptake mechanism (endocytic or not), the interaction of the peptide with the lipid bilayer remains a common issue that needs further investigation. The cell penetrating or antimicrobial properties of exogenous peptides require probably different preliminary interactions with the plasma membrane. Herein, we have employed (31)P NMR, differential scanning calorimetry and CD to study the membrane interaction and perturbation mechanisms of two basic peptides with similar length but distinct charge distribution, penetratin (non-amphipathic) and RL16, a secondary amphipathic peptide. The peptide effects on the thermotropic phase behavior of large multilamellar vesicles of dimyristoylphosphatidylcholine (DMPC), dimyristoylphosphatidylglycerol (DMPG) and dipalmitoleoyl phosphatidylethanolamine (DiPoPE) were investigated. We have found that, even though both peptides are cationic, their interaction with zwitterionic versus anionic lipids is markedly distinct. Penetratin greatly affects the temperature, enthalpy and cooperativity of DMPG main phase transition but does not affect those of DMPC while RL16 presents opposite effects. Additionally, it was found that penetratin induces a negative curvature whereas RL16 induces a positive one, since a decrease in the fluid lamellar to inverted hexagonal phase transition temperature of DiPoPE (T(H)) was observed for penetratin and an increase for RL16. Contrary to penetratin, (31)P NMR of samples containing DMPC MLVs and RL16 shows an isotropic signal indicative of the formation of small vesicles, concomitant with a great decrease in sample turbidity both below and at the phase transition temperature. Opposite effects were also observed on DMPG where both peptides provoke strong aggregation and precipitation. Both CPPs adopt helical structures when contacting with anionic lipids, and possess a dual behavior by either presenting their cationic or hydrophobic domains towards the phospholipid face, depending on the lipid nature (anionic vs zwitterionic, respectively). Surprisingly, the increase of electrostatic interactions at the water membrane interface prevents the insertion of RL16 hydrophobic region in the bilayer, but is essential for the interaction of penetratin. Modulation of amphipathic profiles and charge distribution of CPPs can alter the balance of hydrophobic and electrostatic membrane interaction leading to translocation or and membrane permeabilisation. Penetratin has a relative pure CPP behavior whereas RL16 presents mixed CPP/AMP properties. A better understanding of those processes is essential to unveil their cell translocation mechanism.
Databáze: OpenAIRE
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