Identification and functional characterization of multiple interleukin 12 in amberjack (Seriola dumerili)
| Autor: | Kazuma Hayashi, Kyosuke Araki, Atsushi Yamamoto, Megumi Matsumoto, Hiroaki Suetake |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Fish Proteins
Nocardiaceae 0301 basic medicine medicine.medical_treatment Stimulation Aquatic Science Vaccines Attenuated Interleukin-12 Subunit p35 Microbiology Fish Diseases 03 medical and health sciences Immunity medicine Animals Environmental Chemistry Amino Acid Sequence Amberjack Phylogeny Vaccines Synthetic biology Interleukin-12 Subunit p40 Interleukin 04 agricultural and veterinary sciences General Medicine biology.organism_classification Interleukin-12 Seriola dumerili Perciformes Bacterial vaccine 030104 developmental biology Cytokine Vaccines Inactivated Bacterial Vaccines Immunology 040102 fisheries Interleukin 12 0401 agriculture forestry and fisheries Actinomycetales Infections Sequence Alignment |
| Zdroj: | Fish & Shellfish Immunology. 55:281-292 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2016.05.025 |
| Popis: | Interleukin (IL) -12 is a heterodimeric cytokine mainly produced by monocytes, macrophages, and dendritic cells in mammals. IL-12p70 composed of IL-12p35 and IL-12p40, is known to play a crucial role in promoting cell-mediated immunity (CMI) through Th1 differentiation and IFN-γ production. Although two types of IL-12p35 (p35a, p35b) and three types of IL-12p40 (p40a, p40b and p40c) have been identified in several fish species, the knowledge on functional characteristics of teleost IL-12 is still limited. In the present study, we cloned two types of IL-12p35 and three types of IL-12p40 genes in amberjack and yellowtail, and analyzed their expressions in response to stimulation with Nocardia seriolae in amberjack. As a result, four types of IL-12 (IL-12p35a, p35b, p40a and p40b) and IFN-γ mRNA were increased by live-N. seriolae stimulation but not by formalin-killed N. seriolae, suggesting that four types of IL-12 (p35, p35b, p40a and p40c) participate in promoting CMI. Subsequently, we produced six types of recombinant IL-12p70 (rIL12p70) protein in insect cells. Head kidney leukocytes were cultured with formalin-killed N. seriolae and six types of rIL-12p70 to elucidate the role of amberjack IL-12p70 in induction of CMI. After stimulation, IFN-γ expression was elevated whereas IL-10 expression was suppressed in Head kidney leukocytes stimulated with four types of rIL-12 (p40a/p35a, p40c/p35a, p40a/p35b, p40a/p35b). On the other hand, two types of rIL-12 (p40b/p35a, p40b/p35b) only elicited down regulation of IL-10 expression. These results indicate that all amberjack IL-12p70 isoforms are involved in Th1 -differentiation and promotion of CMI with different manners. Fish IL-12 has a potential for the promising vaccine adjuvant. |
| Databáze: | OpenAIRE |
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