Jacalin-Activated Macrophages Exhibit an Antitumor Phenotype
| Autor: | Luciana Chain Veronez, Maria Cristina Roque-Barreira, Luciana Pereira Ruas, Thais Herrero Geraldino, Claudia Danella Polli, Gabriela Pereira-da-Silva, Fabiana Rossetto de Morais |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Article Subject Nitric Oxide Synthase Type II lcsh:Medicine Breast Neoplasms Biology General Biochemistry Genetics and Molecular Biology Proinflammatory cytokine 03 medical and health sciences HT29 Cells Tumor Microenvironment Macrophage Humans RNA Messenger Tumor microenvironment General Immunology and Microbiology Macrophages lcsh:R NF-kappa B General Medicine Phenotype Gene Expression Regulation Neoplastic 030104 developmental biology Apoptosis Immunology Colonic Neoplasms Cancer research Jacalin MCF-7 Cells MORACEAE Female Signal transduction Plant Lectins Signal Transduction Research Article |
| Zdroj: | BioMed Research International, Vol 2016 (2016) BioMed Research International Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2016/2925657 |
| Popis: | Tumor-associated macrophages (TAMs) have an ambiguous and complex role in the carcinogenic process, since these cells can be polarized into different phenotypes (proinflammatory, antitumor cells or anti-inflammatory, protumor cells) by the tumor microenvironment. Given that the interactions between tumor cells and TAMs involve several players, a better understanding of the function and regulation of TAMs is crucial to interfere with their differentiation in attempts to skew TAM polarization into cells with a proinflammatory antitumor phenotype. In this study, we investigated the modulation of macrophage tumoricidal activities by the lectin jacalin. Jacalin bound to macrophage surface and induced the expression and/or release of mainly proinflammatory cytokines via NF-κB signaling, as well as increased iNOS mRNA expression, suggesting that the lectin polarizes macrophages toward the antitumor phenotype. Therefore, tumoricidal activities of jacalin-stimulated macrophages were evaluated. High rates of tumor cell (human colon, HT-29, and breast, MCF-7, cells) apoptosis were observed upon incubation with supernatants from jacalin-stimulated macrophages. Taken together, these results indicate that jacalin, by exerting a proinflammatory activity, can direct macrophages to an antitumor phenotype. Deep knowledge of the regulation of TAM functions is essential for the development of innovative anticancer strategies. |
| Databáze: | OpenAIRE |
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