Sensory Hairs and Filament Rods in Vestibular Hair Cells of the Waltzing Guinea Pig. Organization and Identification of Actin
| Autor: | Aron Sobin, Åke Flock |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Guinea Pigs
macromolecular substances Cuticular plate Deafness Biology Protein filament Hair Cells Auditory Myosin otorhinolaryngologic diseases medicine Animals Vestibular Hair Cell Actin Inclusion Bodies Vestibular system Stereocilia General Medicine Anatomy Actins Organoids Microscopy Electron medicine.anatomical_structure Otorhinolaryngology Biophysics Vestibule Labyrinth sense organs Nucleus |
| Zdroj: | Acta Oto-Laryngologica. 91:247-254 |
| ISSN: | 1651-2251 0001-6489 |
| DOI: | 10.3109/00016488109138505 |
| Popis: | The waltzing guinea pig suffers from hereditary deafness and vestibular disorder. In vestibular organs, hair cells of Type I develop pathologically and will eventually degenerate. They show fusion of sensory hairs, protrusion of the cuticular plate and contain a rod-shaped inclusion body. With fixation techniques designed to preserve proteins it is shown that this rod has a filamentous substructure reminding one of stereocilia. The packing density of the filaments is similar and circular packing patterns are seen within both structures. However, the rod has an irregular cross-section, as opposed to the circular circumference of stereocilia. The filaments in the rod were identified as containing the protein actin (as those in the stereocilia) by decoration with sub-fragment S-1 of myosin. All filaments in the rod have an identical functional polarity, pointing up from the nucleus towards the cuticular plate. This is contrary to that seen in stereocilia, which have filaments pointing down towards the cuticular plate. It is concluded that the rod is not developed by random polymerization of actin but is the result of co-ordinated assembly reminiscent of that which gives rise to stereocilia. The genetic defect appears to be related to mechanisms which determine the site of nucleation and the functional orientation of actin filaments during development. |
| Databáze: | OpenAIRE |
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