Proteomic Analysis of Bronchoalveolar Lavage Fluid: Effect of Acute Exposure to Diesel Exhaust Particles in Rats
| Autor: | Paul L. Knechtges, Vince Castranova, John A. Lewis, William E. Dennis, Val Vallyathan, K. Murali Krishna Rao |
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| Rok vydání: | 2007 |
| Předmět: |
Proteomics
Pathology medicine.medical_specialty Time Factors Diesel exhaust Health Toxicology and Mutagenesis Inflammation macrophage Lung injury calprotectin complex mixtures diesel Pathogenesis Diesel fuel medicine Animals Lung Chromatography High Pressure Liquid Vehicle Emissions mass spectrometry Dose-Response Relationship Drug medicine.diagnostic_test Chemistry Research Public Health Environmental and Occupational Health Proteins respiratory system Rats respiratory tract diseases Dose–response relationship medicine.anatomical_structure Bronchoalveolar lavage Gene Expression Regulation inflammation Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization SELDI Immunology medicine.symptom Bronchoalveolar Lavage Fluid human activities |
| Zdroj: | Environmental Health Perspectives |
| ISSN: | 1552-9924 0091-6765 |
| DOI: | 10.1289/ehp.9745 |
| Popis: | Background Inhalation of diesel exhaust particles (DEPs) is characterized by lung injury and inflammation, with significant increases in the numbers of polymorphonuclear leukocytes and alveolar macrophages. This influx of cellular infiltrates is associated with the activation of multiple genes, including cytokines and chemokines, and the production of reactive oxygen species. Objective The pathogenesis of the lung injury is not fully understood, but alterations in the presence or abundance of a number of proteins in the lung have been observed. Our objective in this study was to further characterize these changes and to ask whether additional changes could be discerned using modern proteomic techniques. Methods The present study investigates global alterations in the proteome of bronchoalveolar lavage fluid taken from rats 1, 7, or 30 days after exposure to 5, 35, or 50 mg/kg of animal weight of DEPs. Results Analysis by surface-enhanced laser desorption/ionization–time of flight mass spectrometry identified two distinct peaks that appeared as an acute response postexposure at all doses in all animals. We identified these two peaks, with mass to charge ratios (m/z) of 9,100 and 10,100, as anaphylatoxin C3a and calgranulin A by additional mass spectral investigation using liquid chromatography coupled to mass spectrometry. Conclusions With this approach, we found a number of inflammatory response proteins that may be associated with the early phases of inflammation in response to DEP exposure. Further studies are warranted to determine whether serum levels of these proteins could be markers of diesel exhaust exposure in workers. |
| Databáze: | OpenAIRE |
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