Characterization of side-population cells in human normal endometrium
| Autor: | Keiji Kato, Tatsutoshi Nakahata, Asako Yamayoshi, Norio Wake, Momoko Yoshimoto, Takahiro Arima, Kiyoko Kato, Kazuo Asanoma, Satoru Kyo, Sawako Adachi |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Adult
medicine.medical_specialty Cellular differentiation Population Cell Separation CD13 Antigens Biology Endometrium Tetraspanin 29 Antigen Antigens CD Internal medicine medicine Humans Progenitor cell education Cells Cultured education.field_of_study Membrane Glycoproteins Rehabilitation Obstetrics and Gynecology Cell Differentiation Middle Aged Molecular biology Staining Adult Stem Cells medicine.anatomical_structure Endocrinology Reproductive Medicine Female Stem cell Adult stem cell |
| Zdroj: | Human Reproduction. 22:1214-1223 |
| ISSN: | 1460-2350 0268-1161 |
| Popis: | Background It has been proposed that the human endometrium may contain a population of adult stem cells that are responsible for its remarkable regenerative capability. Recently, a subset of stem cells or progenitor cells in adult tissue has been identified as side-population cells (SP cells) displaying low staining with Hoechst 33342 by fluorescence-activated cell sorter (FACS) analysis. In this study, we isolated SP cells from the human endometrium and analysed their properties. Method Endometrial cells were obtained using enzymatic digestion from uterine hysterectomy for the treatment of uterine myoma and stained with Hoechst 33342 dye either alone or in combination with verapamil. The cells were then analysed using FACS. Results SP cells were present among normal human endometrial cells. Most SP cells were enriched in the CD9(-)CD13(-) fraction. These SP cells showed long-term repopulating properties and produced gland (CD9(+))- and stroma (CD13(+))-like cells. CD9(-)CD13(-) cells isolated from the endometrium also generated gland- or stroma-like cells. Conclusions SP cells in the human endometrium can function as progenitor cells. This is the first report of the phenotype of SP cells from normal human endometrial cells. |
| Databáze: | OpenAIRE |
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