Importance of viral genomic composition in modulating glycoprotein content on the surface of influenza virus particles

Autor: Jean-Paul Rolland, Christine Moriscot, Emmanuel Giudice, Bruno Lina, Vincent Moules, Olivier Terrier, Guy Schoehn, Emilie Frobert, Thomas Julien, M. Bouscambert-Duchamp, Yi Pu Lin, Béatrice Riteau, Olivier Ferraris, Alan J. Hay, Daniel Thomas, Alexandra Erny, Matthieu Yver, Manuel Rosa-Calatrava
Přispěvatelé: Virologie et pathologie humaine (VirPath), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Inserm European Associated Laboratory, University of Dundee, Unit for Virus Host-Cell Interactions [Grenoble] (UVHCI), Centre National de la Recherche Scientifique (CNRS)-European Molecular Biology Laboratory [Grenoble] (EMBL)-Université Joseph Fourier - Grenoble 1 (UJF), Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Interactions cellulaires et moléculaires (ICM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), World Health Organization Collaborating Centre Medical Research Council/National Institute of Medical Research, CNRS, Virologie et pathologie humaine ( VirPath ), Université Claude Bernard Lyon 1 ( UCBL ), Unit of Virus Host Cell Interactions ( UVHCI ), Université Joseph Fourier - Grenoble 1 ( UJF ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de biologie structurale ( IBS - UMR 5075 ), Université Joseph Fourier - Grenoble 1 ( UJF ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Grenoble Alpes ( UGA ), Interactions cellulaires et moléculaires ( ICM ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -IFR140-Centre National de la Recherche Scientifique ( CNRS ), Université Joseph Fourier - Grenoble 1 (UJF)-European Molecular Biology Laboratory [Grenoble] (EMBL)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
MESH : Cell Line
viruses
Hemagglutinin Glycoproteins
Influenza Virus
law.invention
MESH: Dogs
Influenza A Virus
H1N1 Subtype
MESH : Dogs
law
Virus Components
MESH: Animals
MESH : Neuraminidase
MESH : Viral Proteins
chemistry.chemical_classification
0303 health sciences
biology
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
030302 biochemistry & molecular biology
Spike density
MESH: Hemagglutinin Glycoproteins
Influenza Virus
MESH: Neuraminidase
Neuraminidase (NA)
Orthomyxoviridae
Cryo-electron microscopy (cryo-EM)
MESH : Influenza A Virus
H1N1 Subtype
MESH : RNA Replicase
Recombinant DNA
MESH: Virion
MESH: Cryoelectron Microscopy
MESH: RNA Replicase
MESH : Influenza A Virus
H3N2 Subtype
Neuraminidase
MESH: Influenza A Virus
H3N2 Subtype
Virus
Cell Line
MESH: Influenza A Virus
H1N1 Subtype
Viral Proteins
03 medical and health sciences
Dogs
Virology
MESH : Virion
Animals
Humans
Polymerase Gene
Haemagglutinin (HA)
030304 developmental biology
MESH: Humans
Host (biology)
Influenza A Virus
H3N2 Subtype
Cryoelectron Microscopy
MESH : Humans
Virion
MESH : Cryoelectron Microscopy
RNA-Dependent RNA Polymerase
biology.organism_classification
MESH: Viral Proteins
Influenza
Viral glycoprotein
MESH: Cell Line
MESH : Hemagglutinin Glycoproteins
Influenza Virus
chemistry
MESH : Animals
Glycoprotein
[ SDV.BBM.BS ] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
Zdroj: Virology
Virology, Elsevier, 2011, 414 (1), pp.51-62. ⟨10.1016/j.virol.2011.03.011⟩
Virology, Elsevier, 2011, 414 (1), pp.51-62. 〈10.1016/j.virol.2011.03.011〉
Virology, 2011, 414 (1), pp.51-62. ⟨10.1016/j.virol.2011.03.011⟩
ISSN: 0042-6822
1096-0341
DOI: 10.1016/j.virol.2011.03.011⟩
Popis: International audience; Despite progress in our knowledge of the internal organisation of influenza virus particles, little is known about the determinants of their morphology and, more particularly, of the actual abundance of structural proteins at the virion level. To address these issues, we used cryo-EM to focus on viral (and host) factors that might account for observed differences in virion morphology and characteristics such as size, shape and glycoprotein (GP) spike density. Twelve recombinant viruses were characterised in terms of their morphology, neuraminidase activity and virus growth. The genomic composition was shown to be important in determining the GP spike density. In particular, polymerase gene segments and especially PB1/PB2 were shown to have a prominent influence in addition to that for HA in determining GP spike density, a feature consistent with a functional link between these virus components important for virus fitness.
Databáze: OpenAIRE
Externí odkaz: