Asymmetric cancer hallmarks in breast tumors on different sides of the body

Autor: Guillermo Urrutia, Javier I. J. Orozco, Luis S. Mayorga, Angela Mathison, Maria Teresita Branham, Emanuel M. Campoy, María Roqué, Francisco E. Gago, Sergio Laurito, Raul Urrutia
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Pathology
lcsh:Medicine
Biochemistry
Metastasis
Epigenesis
Genetic
Cohort Studies
Mathematical and Statistical Techniques
0302 clinical medicine
Lateral Asymmetries
Breast Tumors
Basic Cancer Research
Medicine and Health Sciences
lcsh:Science
Regulation of gene expression
DNA methylation
Multidisciplinary
Chemical Reactions
purl.org/becyt/ford/3.1 [https]
Middle Aged
Bioquímica y Biología Molecular
Chromatin
Nucleic acids
Gene Expression Regulation
Neoplastic
Chemistry
Medicina Básica
Oncology
CpG site
030220 oncology & carcinogenesis
Physical Sciences
Regression Analysis
Epigenetics
Female
purl.org/becyt/ford/3 [https]
DNA modification
Chromatin modification
Statistics (Mathematics)
Research Article
Chromosome biology
Adult
Cell biology
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
Genética Humana
Breast Neoplasms
Biology
Research and Analysis Methods
Methylation
03 medical and health sciences
Breast cancer
Breast Cancer
Genetics
medicine
Humans
Statistical Methods
Aged
Biology and life sciences
Gene Expression Profiling
Carcinoma
lcsh:R
Cancers and Neoplasms
Cancer
DNA
medicine.disease
Gene expression profiling
030104 developmental biology
Cancer research
CpG Islands
lcsh:Q
Gene expression
Mathematics
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
PLoS ONE, Vol 11, Iss 7, p e0157416 (2016)
PLoS ONE
DOI: 10.1371/journal.pone.0157416
Popis: During the last decades it has been established that breast cancer arises through the accumulation of genetic and epigenetic alterations in different cancer related genes. These alterations confer the tumor oncogenic abilities, which can be resumed as cancer hallmarks (CH). The purpose of this study was to establish the methylation profile of CpG sites located in cancer genes in breast tumors so as to infer their potential impact on 6 CH: i.e. sustained proliferative signaling, evasion of growth suppressors, resistance to cell death, induction of angiogenesis, genome instability and invasion and metastasis. For 51 breast carcinomas, MS-MLPA derived-methylation profiles of 81 CpG sites were converted into 6 CH profiles. CH profiles distribution was tested by different statistical methods and correlated with clinical-pathological data. Unsupervised Hierarchical Cluster Analysis revealed that CH profiles segregate in two main groups (bootstrapping 90-100%), which correlate with breast laterality (p = 0.05). For validating these observations, gene expression data was obtained by RealTime-PCR in a different cohort of 25 tumors and converted into CH profiles. This analyses confirmed the same clustering and a tendency of association with breast laterality (p = 0.15). In silico analyses on gene expression data from TCGA Breast dataset from left and right breast tumors showed that they differed significantly when data was previously converted into CH profiles (p = 0.033). We show here for the first time, that breast carcinomas arising on different sides of the body present differential cancer traits inferred from methylation and expression profiles. Our results indicate that by converting methylation or expression profiles in terms of Cancer Hallmarks, it would allow to uncover veiled associations with clinical features. These results contribute with a new finding to the better understanding of breast tumor behavior, and can moreover serve as proof of principle for other bilateral cancers like lung, testes or kidney. Fil: Campoy, Emanuel Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Laurito, Sergio Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Branham, Maria Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Urrutia, Guillermo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Mathison, Angela. Mayo Clinic; Estados Unidos Fil: Gago, Francisco. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Instituto Ginecomamario de Mendoza; Argentina. Hospital Italiano; Argentina Fil: Orozco, Javier. Instituto Ginecomamario de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Hospital Italiano; Argentina Fil: Urrutia, Raúl. Mayo Clinic; Estados Unidos Fil: Mayorga, Luis Segundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Roque Moreno, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Databáze: OpenAIRE
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