MIF mRNA Expression and Soluble Levels in Acute Coronary Syndrome

Autor: Elena Sandoval-Pinto, Héctor Enrique Flores-Salinas, Ilian Janet García-González, José Francisco Muñoz-Valle, Jorge Manuel Pérez-Ibarra, Jorge Ramón Padilla-Gutiérrez, Yeminia Valle, Angélica Valdez-Haro, Emmanuel Valdés-Alvarado
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Cardiology Research and Practice, Vol 2018 (2018)
Cardiology Research and Practice
ISSN: 2090-8016
DOI: 10.1155/2018/9635652
Popis: Acute coronary syndrome (ACS) describes any condition characterized by myocardial ischaemia and reduction in blood flow. The physiopathological process of ACS is the atherosclerosis where MIF operates as a major regulator of inflammation. The aim of this study was to assess the mRNA expression of MIF gene and its serum levels in the clinical manifestations of ACS and unrelated individuals age- and sex-matched with patients as the control group (CG). All samples were run using the conditions indicated in TaqMan Gene Expression Assay protocol. Determination of MIF serum levels were performed by enzyme-linked immunosorbent assay and MIF ELISA Kit. ST-segment elevation myocardial infraction (STEMI) and non-ST-segment elevation myocardial infarction (NSTEMI) showed 0.8 and 0.88, respectively, less expression of MIF mRNA with regard to CG. UA and STEMI presented more expression than NSTEMI 5.23 and 0.68, respectively. Otherwise, ACS patients showed significant higher MIF serum levels (p=0.02) compared with CG. Furthermore, the highest soluble levels of MIF were presented by STEMI (11.21 ng/dL), followed by UA (10.34 ng/dL) and finally NSTEMI patients (8.75 ng/dL); however, the differences were not significant. These novel observations further establish the process of MIF release after cardiovascular events and could support the idea of MIF as a new cardiac biomarker in ACS.
Databáze: OpenAIRE
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