Biochemical Mechanisms, Cross-resistance and Stability of Resistance to Metaflumizone in Plutella xylostella

Autor:	Rumeng Wang, Hong You, Jun Shen, Zhang Shuzhen, Dongyang Li, Zhao Li, Jianhong Li
Rok vydání:	2020
Předmět:	0106 biological sciences Piperonyl butoxide Population Spinosad 01 natural sciences Article resistance Toxicology chemistry.chemical_compound Metaflumizone medicine Plutella xylostella lcsh:Science detoxification enzymes education education.field_of_study Diamondback moth biology Indoxacarb Chlorfenapyr biology.organism_classification 010602 entomology chemistry Insect Science Abamectin lcsh:Q metaflumizone cross-resistance 010606 plant biology & botany medicine.drug
Zdroj:	Insects Volume 11 Issue 5 Insects, Vol 11, Iss 311, p 311 (2020)
ISSN:	2075-4450
Popis:	The diamondback moth, Plutella xylostella (L.) is an important pest of cruciferous crops worldwide. It has developed resistance to many conventional and novel insecticide classes. Metaflumizone belongs to the new chemical class of semicarbazone insecticides. To delay the development of metaflumizone resistance in P. xylostella and to guide insecticide use in the field, the biochemical mechanisms, cross-resistance spectrum, and stability of resistance to metaflumizone were studied in a laboratory-selected resistant strain (metaflu-SEL). Synergism tests with the carboxylesterase inhibitor triphenyl phosphate (TPP), the glutathione S-transferase depletor diethyl maleate (DEM), and the P450 inhibitor piperonyl butoxide(PBO) had no obvious effect on metaflumizone in the metaflu-SEL strain and the susceptible strain (SS) of P. xylostella, with synergism ratios that ranged from 1.02 to 1.86. Biochemical studies revealed that the cytochrome P450-dependent monooxygenase increased only 1.13-fold in the metaflu-SEL strain compared with the UNSEL stain meanwhile, carboxylesterase and glutathione S-transferase activity showed no difference. These results suggest that these detoxification enzymes may be not actively involved in metaflumizone resistance. Furthermore, the metaflu-SEL population showed a moderate level of cross-resistance to indoxacarb (11.63-fold), but only very low cross-resistance to spinosad (1.75-fold), spinetoram (3.52-fold), abamectin (2.81-fold), beta-cypermethrin (0.71-fold), diafenthiuron (0.79-fold), chlorantraniliprole (2.16-fold), BT (WG-001) (3.34-fold), chlorfenapyr (0.49-fold), and chlorfluazuron (0.97-fold). Moreover, metaflumizone resistance decreased from 1087.85- to 1.23-fold in the metaflu-SEL strain after 12 generations without exposure to metaflumizone. These results are useful for formulating insecticide resistance management strategies to control P. xylostella and to delay the development of metaflumizone resistance in the field.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fef79927c81606659ddc6ac693f29d6b https://doi.org/10.3390/insects11050311 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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