An iron stable isotope comparison between human erythrocytes and plasma
| Autor: | Kirsten van Zuilen, Hans-Peter Gschwind, Alan Slade, Daniel Kaufmann, Sylvie Stitah, Piet Swart, Friedhelm von Blanckenburg, Dietmar G. Schmid, Marcus Oelze |
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| Přispěvatelé: | Geology and Geochemistry |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Erythrocytes Adolescent Biophysics Analytical chemistry Mass spectrometry Biochemistry Redox Biomaterials Plasma Young Adult Blood plasma Humans 550 Earth sciences & geology chemistry.chemical_classification Isotope biology Stable isotope ratio Metals and Alloys Reproducibility of Results Middle Aged Iron Isotopes Ferritin chemistry Chemistry (miscellaneous) Transferrin biology.protein Composition (visual arts) |
| Zdroj: | von Blanckenburg, F, Oelze, M, Schmid, D G, van Zuilen, K, Gschwind, H-P, Slade, A J, Stitah, S, Kaufmann, D & Swart, P 2014, ' An iron stable isotope comparison between human erythrocytes and plasma ', Metallomics, vol. 6, no. 11, pp. 2052-2061 . https://doi.org/10.1039/c4mt00124a Metallomics von Blanckenburg, Friedhelm; Oelze, Marcus; Schmid, Dietmar G.; van Zuilen, Kirsten; Gschwind, Hans-Peter; Slade, Alan J.; Stitah, Sylvie; Kaufmann, Daniel; Swart, Piet (2014). An iron stable isotope comparison between human erythrocytes and plasma. Metallomics, 6(11), pp. 2052-2061. Royal Society of Chemistry 10.1039/C4MT00124A Metallomics, 6(11), 2052-2061. Royal Society of Chemistry |
| ISSN: | 1756-5901 |
| DOI: | 10.1039/c4mt00124a |
| Popis: | We present precise iron stable isotope ratios measured by multicollector-ICP mass spectrometry (MC-ICP-MS) of human red blood cells (erythrocytes) and blood plasma from 12 healthy male adults taken during a clinical study. The accurate determination of stable isotope ratios in plasma first required substantial method development work, as minor iron amounts in plasma had to be separated from a large organic matrix prior to mass-spectrometric analysis to avoid spectroscopic interferences and shifts in the mass spectrometer's mass-bias. The (56)Fe/(54)Fe ratio in erythrocytes, expressed as permil difference from the "IRMM-014" iron reference standard (δ(56/54)Fe), ranges from -3.1‰ to -2.2‰, a range typical for male Caucasian adults. The individual subject erythrocyte iron isotope composition can be regarded as uniform over the 21 days investigated, as variations (±0.059 to ±0.15‰) are mostly within the analytical precision of reference materials. In plasma, δ(56/54)Fe values measured in two different laboratories range from -3.0‰ to -2.0‰, and are on average 0.24‰ higher than those in erythrocytes. However, this difference is barely resolvable within one standard deviation of the differences (0.22‰). Taking into account the possible contamination due to hemolysis (iron concentrations are only 0.4 to 2 ppm in plasma compared to approx. 480 ppm in erythrocytes), we model the pure plasma δ(56/54)Fe to be on average 0.4‰ higher than that in erythrocytes. Hence, the plasma iron isotope signature lies between that of the liver and that of erythrocytes. This difference can be explained by redox processes involved during cycling of iron between transferrin and ferritin. |
| Databáze: | OpenAIRE |
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