Inhibition of the hemorrhagic and proteolytic activities of Lansberg's hognose pit viper (Porthidium lansbergii hutmanni) venom by opossum (Didelphis marsupialis) serum: isolation of Didelphis marsupialis 0.15Dm fraction on DEAE-cellulose chromatography

Autor:	Irma Fernández, María E. Pineda, Amalid Estrella, Héctor Scannone, Irma Aguilar, María E. Girón, Alba M. Vargas, Elda E. Sánchez, Alexis Rodríguez-Acosta
Rok vydání:	2008
Předmět:	Male Serum Didelphis Proteolysis Immunology Snake Bites Venom Hemorrhage Biology Chemical Fractionation Toxicology complex mixtures Chromatography DEAE-Cellulose Porthidium lansbergii Mice Opossum Hognose Crotalid Venoms medicine Immunology and Allergy Animals Pharmacology medicine.diagnostic_test Pit viper General Medicine Opossums biology.organism_classification Molecular biology Snake venom Peptide Hydrolases
Zdroj:	Immunopharmacology and immunotoxicology. 30(4)
ISSN:	1532-2513
Popis:	Earlier studies have revealed the ability of sera from several mammals to neutralize the toxic effects of snake venom. The Venezuelan opossum (Didelphis marsupialis) is one that has been found to inhibit hemorrhagic and proteolytic activities of venoms from many species of snakes. In this article it is shown that the opossum sera and its 0.15DM fraction were able to completely neutralize both hemorrhagic and hydrolysis (proteolysis) of casein effects induced by venom of the Lansberg's hognose pit viper (Porthidium lansbergii hutmanni). We have used DEAE-cellulose ion exchange chromatography to collect protein fractions from D. marsupialis sera which were able to defend mice from the lethal effects of P.l. hutmanni venom. The fractions separated were homogeneous by conventional electrophoresis using SDS-PAGE. The protein bands obtained contained molecular weights of approximately 6 to 220 kDa. These results revealed the presence of proteases inhibitors in the opossum sera fractions and the inhibition of venom activity by opossum sera suggesting a reciprocal adaptation at the molecular level.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fef32ed5d6d4b8e164bec944fec09cda https://pubmed.ncbi.nlm.nih.gov/18800269 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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