Monoclonal antibodies defining shared human macrophage-endothelial antigens
| Autor: | Richard A. Ashmun, J. C. Burrows, Alisa E. Koch, A. Thomas Look, Peter H. Domer, S. Joseph Leibovich |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Myeloid
Immunogen medicine.drug_class Cross Reactions Monoclonal antibody medicine.disease_cause Cross-reactivity Pathology and Forensic Medicine Arthritis Rheumatoid Mice Antigen medicine Tumor Cells Cultured Macrophage Animals Humans Antigens Molecular Biology Mice Inbred BALB C biology Chemistry Macrophages Antibodies Monoclonal Cell Biology General Medicine 3T3 Cells Molecular biology medicine.anatomical_structure Cell culture Immunology biology.protein Endothelium Vascular Antibody |
| Zdroj: | Pathobiology : journal of immunopathology, molecular and cellular biology. 60(2) |
| ISSN: | 1015-2008 |
| Popis: | We have selected several monoclonal antibodies (mAbs) producing using human rheumatoid arthritis (RA) synovial macrophages (m phi s) as immunogen. Of these, mAbs 8H2, 10G7 and 10G9 showed cross reactivity with endothelium, suggesting common antigens between these cell types. We have determined the spectrum of reactivity of these mAbs on hematopoietic cell lines, peripheral blood cells, and inflammatory and non-inflammatory tissues by immunohistochemistry. MAb 8H2 does not react with the myeloid cell lines HL60 (myelocytic), U937 (histiocytic lymphoma), and K562 (erythroleukemia), or with peripheral blood cells. In normal and inflamed tissue sections, mAb 8H2 reacts with m phi s and endothelial cells. In contrast, mAb 10G7 does not react with peripheral blood cells, but reacts with HL60, U937, and K562 cell lines, as well as with m phi s and endothelial cells in inflamed and noninflamed tissues. MAb 10G9 does not react with myeloid cell lines, but reacts with monocytes and platelets in peripheral blood. In both normal and inflamed tissues, mAb 10G9 reacts with m phi s and endothelial cells. The antigens identified by these three mAbs were characterized biochemically, by enzymatic digestion of RA synovial tissue m phi s followed by a cellular ELISA, as well as by reactivity of the mAbs with NIH-3T3 cells genetically engineered to express known myeloid antigens. These mAbs reacted with protein or glycoprotein antigens distinct from the known myeloid antigens CD13, CD14, CD33, CD34, CD36, and c-fms. These mAbs should prove to be a valuable tool for studying m phi s and endothelial cells and their shared antigenic determinants. |
| Databáze: | OpenAIRE |
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