Cutibacterium avidum is phylogenetically diverse with a subpopulation being adapted to the infant gut
Autor: | Clarissa Schwab, Christophe Lacroix, Vera Bunesova, Christian Braegger, Jiri Killer, Vanesa Natalin Rocha Martin, Evelyn Voney |
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Rok vydání: | 2019 |
Předmět: |
Bifidobacterium longum
ved/biology.organism_classification_rank.species Bifidobacterium longum subspecies infantis Biology Gut flora digestive system Applied Microbiology and Biotechnology Microbiology Carbon utilization Feces 03 medical and health sciences chemistry.chemical_compound Polysaccharides Humans Phylogeny Ecology Evolution Behavior and Systematics 030304 developmental biology Bifidobacterium 0303 health sciences Bifidobacterium bifidum Milk Human Phylogenetic tree 030306 microbiology ved/biology Genetic Variation Infant Sequence Analysis DNA Propionibacteriaceae biology.organism_classification Adaptation Physiological Gastrointestinal Microbiome chemistry Genes Bacterial Galactose Microbial Interactions Propionates Genome Bacterial |
Zdroj: | Systematic and Applied Microbiology. 42:506-516 |
ISSN: | 0723-2020 |
Popis: | The infant gut harbors a diverse microbial community consisting of several taxa whose persistence depends on adaptation to the ecosystem. In healthy breast-fed infants, the gut microbiota is dominated by Bifidobacterium spp.. Cutibacterium avidum is among the initial colonizers, however, the phylogenetic relationship of infant fecal isolates to isolates from other body sites, and C. avidum carbon utilization related to the infant gut ecosystem have been little investigated. In this study, we investigated the phylogenetic and phenotypic diversity of 28 C. avidum strains, including 16 strains isolated from feces of healthy infants. We investigated the in vitro capacity of C. avidum infant isolates to degrade and consume carbon sources present in the infant gut, and metabolic interactions of C. avidum with infant associated Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum. Isolates of C. avidum showed genetic heterogeneity. C. avidum consumed d- and l-lactate, glycerol, glucose, galactose, N-acetyl-d-glucosamine and maltodextrins. Alpha-galactosidase- and β-glucuronidase activity were a trait of a group of non-hemolytic strains, which were mostly isolated from infant feces. Beta-glucuronidase activity correlated with the ability to ferment glucuronic acid. Co-cultivation with B. infantis and B. bifidum enhanced C. avidum growth and production of propionate, confirming metabolic cross-feeding. This study highlights the phylogenetic and functional diversity of C. avidum, their role as secondary glycan degraders and propionate producers, and suggests adaptation of a subpopulation to the infant gut. |
Databáze: | OpenAIRE |
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