Spatially-resolved nanometer-scale measurement of cartilage extracellular matrix mobility
| Autor: | Brittany D. Partain, Kyle D. Allen, Mythreyi Unni, Suresh Narayanan, Qingteng Zhang, Carlos M. Rinaldi-Ramos, Jessica Aldrich |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Length scale Cartilage Articular Biomedical Engineering Matrix (biology) Article Extracellular matrix 03 medical and health sciences 0302 clinical medicine Rheumatology Dynamic light scattering Synovial Fluid medicine Synovial fluid Animals Orthopedics and Sports Medicine 030203 arthritis & rheumatology Chemistry Cartilage Spatially resolved Spectrum Analysis Extracellular Matrix 030104 developmental biology medicine.anatomical_structure Biophysics Nanometre Cattle |
| Zdroj: | Osteoarthritis Cartilage |
| ISSN: | 1522-9653 |
| Popis: | OBJECTIVE: Tissues have complex structures, comprised of solid and fluid phases. Improved understanding of interactions between joint fluid and extracellular matrix (ECM) is required in models of cartilage mechanics. X-ray photon correlation spectroscopy (XPCS) directly measures nanometer-scale dynamics, and thus can provide insight into biofluid-biosolid interactions in cartilage. This study applies XPCS to evaluate dynamic interactions between intact cartilage and biofluids. DESIGN: Cartilage biopsies were collected from bovine femoral condyles. During XPCS measurements, cartilage samples were exposed to different fluids: deionized water, PBS, synovial fluid, or sonicated synovial fluid. ECM-biofluid interactions were also assessed at different length scales and different depths from the cartilage surface. RESULTS: Using SA-XPCS, cartilage ECM mobility was detected at length scales from 50 to 207 nm. As length scale decreased, time scale for autocorrelation decay decreased, suggesting smaller ECM components are more mobile. ECM dynamics were slowed by dehydrating the sample, demonstrating XPCS assesses matrix mobility in hydrated environments. At all length scales, the matrix was more mobile in deionized water and slowest in synovial fluid. Using the 207 nm length scale assessment, ECM dynamics in synovial fluid were fastest at the cartilage surface and progressively slowed as depth into the sample increased, demonstrating XPCS can assess spatial distribution of ECM dynamics. Finally, ECM mobility increased for sonicated synovial fluid. CONCLUSIONS: This study demonstrates the potential of XPCS to provide unique insights into nanometer-scale cartilage ECM mobility in a spatially resolved manner and illustrates the importance of biosolid-biofluid interactions in dictating dynamics of ECM components. |
| Databáze: | OpenAIRE |
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