Cell–cell and cell–extracellular matrix adhesions cooperate to organize actomyosin networks and maintain force transmission during dorsal closure
| Autor: | Teresa Zulueta-Coarasa, Guy Tanentzapf, Emily Lostchuck, Kaitlyn M. L. Cramb, Katharine Goodwin, Rodrigo Fernandez-Gonzalez |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Morphogenesis Embryonic Development Myosins Matrix (biology) Biology Cell-Matrix Junctions Extracellular matrix 03 medical and health sciences Cell Movement Myosin Cell Adhesion Animals Drosophila Proteins Cell Interactions Cell adhesion Molecular Biology Actin Actomyosin Articles Cell Biology Cadherins Actin cytoskeleton Actins Dorsal closure Extracellular Matrix Cell biology Actin Cytoskeleton 030104 developmental biology Drosophila Epidermis |
| Zdroj: | Molecular Biology of the Cell |
| ISSN: | 1939-4586 1059-1524 |
| Popis: | Cell–extracellular matrix (ECM) and cell–cell adhesion are interdependent during dorsal closure in the fly. Cell–ECM adhesion is required for normal myosin dynamics and organization of both cell–cell adhesions and actin networks during dorsal closure. Loss of cell–cell adhesion affects cell–ECM adhesion and tissue biomechanics. Tissue morphogenesis relies on the coordinated action of actin networks, cell–cell adhesions, and cell–extracellular matrix (ECM) adhesions. Such coordination can be achieved through cross-talk between cell–cell and cell–ECM adhesions. Drosophila dorsal closure (DC), a morphogenetic process in which an extraembryonic tissue called the amnioserosa contracts and ingresses to close a discontinuity in the dorsal epidermis of the embryo, requires both cell–cell and cell–ECM adhesions. However, whether the functions of these two types of adhesions are coordinated during DC is not known. Here we analyzed possible interdependence between cell–cell and cell–ECM adhesions during DC and its effect on the actomyosin network. We find that loss of cell–ECM adhesion results in aberrant distributions of cadherin-mediated adhesions and actin networks in the amnioserosa and subsequent disruption of myosin recruitment and dynamics. Moreover, loss of cell–cell adhesion caused up-regulation of cell–ECM adhesion, leading to reduced cell deformation and force transmission across amnioserosa cells. Our results show how interdependence between cell–cell and cell–ECM adhesions is important in regulating cell behaviors, force generation, and force transmission critical for tissue morphogenesis. |
| Databáze: | OpenAIRE |
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