Hyaluronic acid nanoparticle-encapsulated microRNA-125b repolarizes tumor-associated macrophages in pancreatic cancer
Autor: | Mansoor M. Amiji, Gerardo G. Mackenzie, Neha N. Parayath, Brian Hong |
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Rok vydání: | 2021 |
Předmět: |
medicine.medical_treatment
Medical Biotechnology Biomedical Engineering Medicine (miscellaneous) Bioengineering Development Adenocarcinoma Transfection intraperitoneal administration KPC mice Vaccine Related Pancreatic Cancer chemistry.chemical_compound Mice Rare Diseases Pancreatic cancer Hyaluronic acid PEG ratio Tumor-Associated Macrophages pancreatic adenocarcinoma Genetics medicine Nanotechnology Macrophage Animals General Materials Science Nanoscience & Nanotechnology Hyaluronic Acid Cancer hyaluronic acid-poly(ethylene imine) nanoparticles microRNA transfection technology industry and agriculture Immunotherapy medicine.disease In vitro Pancreatic Neoplasms MicroRNAs Orphan Drug medicine.anatomical_structure chemistry Cancer research Nanoparticles Digestive Diseases Pancreas Biotechnology Physical Chemistry (incl. Structural) Research Article |
Zdroj: | Nanomedicine (Lond) Nanomedicine (London, England), vol 16, iss 25 |
ISSN: | 1748-6963 |
Popis: | Aim: To investigate a novel strategy to target tumor-associated macrophages and reprogram them to anantitumor phenotype in pancreatic adenocarcinoma (PDAC). Methods: M2 peptideswere conjugated to HA-PEG/HA-PEI polymer to form self-assembled nanoparticles with miR-125b. The efficacy of HA-PEI/PEG-M2peptide nanoparticles in pancreatic tumors from LSL-KrasG12D/+, LSL-Trp53R172H/+, Pdx1-Cre genetically engineered micewas evaluated. Results:In vitro M2 macrophage-specific delivery of targeted nanoformulationswas demonstrated. Intraperitoneal administration of M2-targeted nanoparticles showed preferential accumulation in the pancreas of KPC-PDAC mice and an abovefourfold increase in the M1-to-M2 macrophage ratio compared with transfection with scrambled miR. Conclusion: M2-targeted HA-PEI/PEG nanoparticles with miR-125b can transfect tumor-associated macrophages in pancreatic tissues and may have implications for PDAC immunotherapy. |
Databáze: | OpenAIRE |
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