Hyaluronic acid nanoparticle-encapsulated microRNA-125b repolarizes tumor-associated macrophages in pancreatic cancer

Autor: Mansoor M. Amiji, Gerardo G. Mackenzie, Neha N. Parayath, Brian Hong
Rok vydání: 2021
Předmět:
medicine.medical_treatment
Medical Biotechnology
Biomedical Engineering
Medicine (miscellaneous)
Bioengineering
Development
Adenocarcinoma
Transfection
intraperitoneal administration
KPC mice
Vaccine Related
Pancreatic Cancer
chemistry.chemical_compound
Mice
Rare Diseases
Pancreatic cancer
Hyaluronic acid
PEG ratio
Tumor-Associated Macrophages
pancreatic adenocarcinoma
Genetics
medicine
Nanotechnology
Macrophage
Animals
General Materials Science
Nanoscience & Nanotechnology
Hyaluronic Acid
Cancer
hyaluronic acid-poly(ethylene imine) nanoparticles
microRNA transfection
technology
industry
and agriculture
Immunotherapy
medicine.disease
In vitro
Pancreatic Neoplasms
MicroRNAs
Orphan Drug
medicine.anatomical_structure
chemistry
Cancer research
Nanoparticles
Digestive Diseases
Pancreas
Biotechnology
Physical Chemistry (incl. Structural)
Research Article
Zdroj: Nanomedicine (Lond)
Nanomedicine (London, England), vol 16, iss 25
ISSN: 1748-6963
Popis: Aim: To investigate a novel strategy to target tumor-associated macrophages and reprogram them to anantitumor phenotype in pancreatic adenocarcinoma (PDAC). Methods: M2 peptideswere conjugated to HA-PEG/HA-PEI polymer to form self-assembled nanoparticles with miR-125b. The efficacy of HA-PEI/PEG-M2peptide nanoparticles in pancreatic tumors from LSL-KrasG12D/+, LSL-Trp53R172H/+, Pdx1-Cre genetically engineered micewas evaluated. Results:In vitro M2 macrophage-specific delivery of targeted nanoformulationswas demonstrated. Intraperitoneal administration of M2-targeted nanoparticles showed preferential accumulation in the pancreas of KPC-PDAC mice and an abovefourfold increase in the M1-to-M2 macrophage ratio compared with transfection with scrambled miR. Conclusion: M2-targeted HA-PEI/PEG nanoparticles with miR-125b can transfect tumor-associated macrophages in pancreatic tissues and may have implications for PDAC immunotherapy.
Databáze: OpenAIRE
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