A pH shift induces high-titer liamocin production in Aureobasidium pullulans

Autor:	Katharina Saur, Till Tiso, Oliver Brumhard, Heiko Hayen, Karen Scholz
Rok vydání:	2019
Předmět:	Nitrogen Applied Microbiology and Biotechnology High-performance liquid chromatography Melanin Industrial Microbiology Surface-Active Agents 03 medical and health sciences chemistry.chemical_compound Bioreactors Sugar Alcohols Ascomycota Polyol Arabitol medicine Mannitol 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chromatography biology 030306 microbiology Pullulan General Medicine Hydrogen-Ion Concentration biology.organism_classification Culture Media Aureobasidium pullulans chemistry Batch Cell Culture Techniques Fermentation Oils Biotechnology medicine.drug
Zdroj:	Applied Microbiology and Biotechnology. 103:4741-4752
ISSN:	1432-0614 0175-7598
DOI:	10.1007/s00253-019-09677-3
Popis:	Liamocins are biosurfactants produced by the fungus Aureobasidium pullulans. A. pullulans belongs to the black yeasts and is known for its ability to produce pullulan and melanin. However, the production of liamocins has not been investigated intensively. Initially, HPLC methods for the quantification of liamocin and the identification of liamocin congeners were established. Eleven congeners could be detected, differing in the polyol head groups arabitol or mannitol. In addition, headless molecules, so-called exophilins, were also identified. The HPLC method reported here allows quick and reliable quantification of all identified congeners, an often-overlooked prerequisite for the investigation of valuable product formation. Liamocin synthesis was optimized during cultivation in lab-scale fermenters. While the pH can be kept constant, the best strategy for liamocin synthesis consists of a growth phase at neutral pH and a subsequent production phase induced by a manual pH shift to pH 3.5. Finally, combining increased nitrogen availability with a pulsed fed-batch fermentation, cell growth, and liamocin titers could be enhanced. Here, the maximal titers of above 10 g/L that were reached are the highest reported to date for liamocin synthesis using A. pullulans in lab-scale fermenters.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fee3eee2265adde3b9aba5f345a57d2b https://doi.org/10.1007/s00253-019-09677-3 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.