Identification of Immunogenic Epitopes by MS/MS
| Autor: | Moreno Di Marco, Hans-Georg Rammensee, Janet Kerstin Peper |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Cancer Research Immunogenicity T cell Cell Peptide Biology medicine.disease_cause Epitope Malignant transformation Autoimmunity Neoplasm Proteins 03 medical and health sciences Epitopes 030104 developmental biology medicine.anatomical_structure Oncology chemistry Tandem Mass Spectrometry Immunology medicine Humans Gene |
| Zdroj: | Cancer journal (Sudbury, Mass.). 23(2) |
| ISSN: | 1540-336X |
| Popis: | The interrogation of cell surface–presented immunogenic epitopes is of great importance to differentiate diseased cells in consequence to malignant transformation or viral infections. On the basis of this knowledge, next-generation immunotherapies against cancers, autoimmunity, or infectious diseases can be developed. The identification of altered peptide repertoires of transformed cells renders mass spectrometry–based analysis indispensable. This is evident considering the low correlation of gene or protein expression alterations, respectively, with changes in the peptide repertoire rendering those analyses less informative. Nevertheless, immunogenicity of peptides appearing to be exclusively found on diseased cells has to be finally proven in T cell–based assays. This review highlights the capabilities and limitations of mass spectrometry in the identification of entire immunopeptidomes, as well as individual potential immunogenic epitopes with a strong focus on cancer. Furthermore, an overview of state-of-the-art immunogenicity screens is presented. |
| Databáze: | OpenAIRE |
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