Phenolic profile, antioxidant capacity and antimicrobial activity of nettle leaves extracts obtained by advanced extraction techniques

Autor: Verica Dragović-Uzelac, Sonja Smole Možina, Maja Repajić, Valentina Malin, Zoran Zorić, Ivona Elez Garofulić, Sandra Pedisić, Meta Sterniša
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Antioxidant
medicine.medical_treatment
Pharmaceutical Science
antioxidant capacity
High-performance liquid chromatography
Antioxidants
Analytical Chemistry
UPLC MS2
chemistry.chemical_compound
Ingredient
QD241-441
Anti-Infective Agents
Pseudomonas fragi
Drug Discovery
pressurized liquid extraction
Food science
Microwaves
Chromatography
High Pressure Liquid
biology
Chemistry
protimikrobno delovanje
Urtica dioica
microwave-assisted extraction
Antimicrobial
Anti-Bacterial Agents
nettle leaves
advanced extraction techniques
plant polyphenols
ORAC
antimicrobial activity
rastlinski izvlečki
Chemistry (miscellaneous)
Molecular Medicine
fenolne spojine
Article
ekstrakcijske metode
Phenols
kopriva
medicine
Physical and Theoretical Chemistry
udc:547.9:582.635.8:66.061
UPLC MS$^2$
Flavonoids
ekstrakcija
Plant Extracts
Organic Chemistry
Extraction (chemistry)
antioksidativni potencial
biology.organism_classification
Plant Leaves
Polyphenol
Zdroj: Molecules, vol. 26, no. 20, 6153, 2021.
Molecules
Molecules, Vol 26, Iss 6153, p 6153 (2021)
Volume 26
Issue 20
ISSN: 1420-3049
Popis: Nettle is a widely known plant whose high biological activity and beneficial medicinal effects are attributed to various bioactive compounds, among which polyphenols play an important role. In order to isolate polyphenols and preserve their properties, advanced extraction techniques have been applied to overcome the drawbacks of conventional ones. Therefore, microwave-assisted extraction (MAE) has been optimized for the isolation of nettle leaves polyphenols and it was compared to pressurized liquid extraction (PLE) and conventional heat-reflux extraction (CE). The obtained extracts were analyzed for their individual phenolic profile by UPLC MS2 and for their antioxidant capacity by ORAC assay. MAE proved to be the more specific technique for the isolation of individual phenolic compounds, while PLE produced extracts with higher amount of total phenols and higher antioxidant capacity. Both techniques were more effective compared to CE. PLE nettle extract showed antimicrobial activity against bacteria, especially against Gram-negative Pseudomonas fragi ATCC 4973 and Campylobacter jejuni NCTC 11168 strains. This suggests that PLE is suitable for obtaining a nettle extract with antioxidant and antimicrobial potential, which as such has great potential for use as a value-added ingredient in the food and pharmaceutical industry.
Databáze: OpenAIRE
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